An in vitro model for determining tumor cell migration under metabolic gradients
An in vitro model for determining tumor cell migration under metabolic gradients
复制标题
代谢梯度下确定肿瘤细胞迁移的体外模型
DOI：
10.1007/978-3-319-91287-5_32
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Takahashi E
中科院分区：
文献类型：
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作者：
Tsuruno Y;Okubo K;Fujiwara T;Yamaoka Y;Takahashi E
为了回答MDA-MB-231细胞是否根据代谢线索（如O2浓度梯度）主动迁移的问题，我们开发了差距盖玻片（GCG），以在体外培养的细胞组织中产生代谢梯度。由于GCG利用细胞的代谢活动来建立代谢梯度，因此必须增加GCG下的细胞数量。然而，细胞密度的增加增加了细胞之间碰撞的机会，这是确定细胞迁移方向性的严重人为因素。在本研究中，通过将我们的GCG与传统的伤口愈合测定相结合，我们成功地大大减少了细胞碰撞引起的伪影。使用这种技术，我们证明了一个单向迁移的MDA-MB-231细胞的代谢梯度下产生的GCG，即使在21%O2。
To answer the question whether MDA-MB-231 cells actively migrate according to metabolic cues, such as gradients of O2concentration, we developed the gap cover glass (GCG) to produce metabolic gradients in cultured cell tissue in vitro. Because the GCG utilizes metabolic activities of the cell to establish metabolic gradients, the number of cells under the GCG must be increased. However, an increase in cell density increases the chance of collision between cells, which is a serious artifact in determining the directionality of cell migration. In the present study, by combining our GCG with the conventional wound healing assay, we succeeded in substantially reducing artifacts arising from cell collision. Using this technique, we demonstrated a unidirectional migration of MDA-MB-231 cells under metabolic gradients produced by the GCG, even at 21% O2.