Respiratory and Mayer wave-related discharge patterns of raphe and pontine neurons change with vagotomy
Respiratory and Mayer wave-related discharge patterns of raphe and pontine neurons change with vagotomy
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DOI：
10.1152/japplphysiol.01324.2009
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发表时间：
2010-07-01
影响因子：
3.3
通讯作者：
Dick, T. E.
中科院分区：
文献类型：
--
作者：
Morris, K. F.;Nuding, S. C.;Dick, T. E.
莫里斯 KF、努丁 SC、西格斯 LS、贝基 DM、香农 R、林赛 BG、迪克 TE。中缝和脑桥神经元的呼吸和迈耶波相关放电模式随着迷走神经切断而改变。 J Appl Physiol 109: 189-202, 2010。首次发表于 2010 年 4 月 1 日； doi:10.1152/japplphyol.01324.2009.-以前的模型将迷走神经切断术后脑桥神经元呼吸调节的变化归因于吸气驱动传出副本的肺部牵张受体“门控”的丧失。最近，我们的小组证实，尽管样本的平均峰值和平均放电率没有增加，但在迷走神经切断术后，脑桥神经元会改变放电模式并变得更加呼吸调节（Dick et al., J Physiol 586: 4265-4282, 2008）。由于中缝神经元也是脑干呼吸网络的组成部分，因此我们测试了以下假设：迷走神经切断术后，中缝神经元的呼吸调节增加，并且其放电模式的变化与肺充气抑制期间脑桥神经元的变化相似。在去大脑猫的迷走神经切断术前后同时记录中缝和脑桥神经元。在迷走神经切断术之前，95 个中缝神经元中的 14% 在通过抑制肺充气而延长的单个呼吸周期中活动增加； 13% 的人表现出活动减少。迷走神经切断术后，呼吸调节的平均指数 (eta(2)) 增加（0.05 +/- 0.10 至 0.12 +/- 0.18 SD；学生配对 t 检验，P < 0.01）。时间序列和频域分析确定了脑桥和中缝神经元放电率调制，其节律为 0.1 Hz，与血压 Mayer 波一致。这些“迈耶波相关振荡”（MWRO）与中枢呼吸驱动相结合，并在迷走神经切断后与中枢呼吸节律同步（10只动物中的7只）。互相关分析确定了 360 对神经元中 52 对具有 MWRO 的功能连接。总的来说，结果表明分布式网络参与 MWRO 的生成以及呼吸和血管舒缩节律的协调。
Morris KF, Nuding SC, Segers LS, Baekey DM, Shannon R, Lindsey BG, Dick TE. Respiratory and Mayer wave-related discharge patterns of raphe and pontine neurons change with vagotomy. J Appl Physiol 109: 189-202, 2010. First published April 1, 2010; doi:10.1152/japplphysiol.01324.2009.-Previous models have attributed changes in respiratory modulation of pontine neurons after vagotomy to a loss of pulmonary stretch receptor "gating" of an efference copy of inspiratory drive. Recently, our group confirmed that pontine neurons change firing patterns and become more respiratory modulated after vagotomy, although average peak and mean firing rates of the sample did not increase (Dick et al., J Physiol 586: 4265-4282, 2008). Because raphe neurons are also elements of the brain stem respiratory network, we tested the hypotheses that after vagotomy raphe neurons have increased respiratory modulation and that alterations in their firing patterns are similar to those seen for pontine neurons during withheld lung inflation. Raphe and pontine neurons were recorded simultaneously before and after vagotomy in decerebrated cats. Before vagotomy, 14% of 95 raphe neurons had increased activity during single respiratory cycles prolonged by withholding lung inflation; 13% exhibited decreased activity. After vagotomy, the average index of respiratory modulation (eta(2)) increased (0.05 +/- 0.10 to 0.12 +/- 0.18 SD; Student's paired t-test, P < 0.01). Time series and frequency domain analyses identified pontine and raphe neuron firing rate modulations with a 0.1-Hz rhythm coherent with blood pressure Mayer waves. These "Mayer wave-related oscillations" (MWROs) were coupled with central respiratory drive and became synchronized with the central respiratory rhythm after vagotomy (7 of 10 animals). Cross-correlation analysis identified functional connectivity in 52 of 360 pairs of neurons with MWROs. Collectively, the results suggest that a distributed network participates in the generation of MWROs and in the coordination of respiratory and vasomotor rhythms.