The molecular characterization and expression of heat shock protein 90 (Hsp90) and 26 (Hsp26) cDNAs in sea cucumber (Apostichopus japonicus)
The molecular characterization and expression of heat shock protein 90 (Hsp90) and 26 (Hsp26) cDNAs in sea cucumber (Apostichopus japonicus)
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DOI：
10.1007/s12192-011-0260-z
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发表时间：
2011-04
影响因子：
3.8
通讯作者：
Huan Zhao;Hongsheng Yang;Heling Zhao;Muyan Chen;Tianming Wang
中科院分区：
文献类型：
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作者：
Huan Zhao;Hongsheng Yang;Heling Zhao;Muyan Chen;Tianming Wang
热休克蛋白（HSP）是一个蛋白质家族，其表达在响应环境应激时增强。利用表达序列标签和cDNA末端快速扩增技术克隆了仿刺参hsp 90和hsp 26基因。Aphsp 90和Aphsp 26的全长cDNA分别含有3，458和1，688个核苷酸，编码720和236个氨基酸。多重序列比对表明，ApHsp 90和ApHsp 26的氨基酸序列与斑马鱼、蚂蚁、橡实蠕虫等的Hsp 90和小分子SHP（sHSPs）序列具有较高的同源性，与Hsp 90和sHSPs具有相同的结构特征。采用实时荧光定量PCR技术研究了这两个基因在热处理条件下的表达谱。结果发现，这两个基因的信使RNA（mRNA）转录本A.在正常条件下和热休克条件下，不同组织中的japonicusgene存在差异，并且与其他组织相比，这两个基因的mRNA表达在肠中较高。热休克显著提高Aphsp 90和Aphsp 26 mRNA的表达，且呈时间和温度依赖性。结果表明，Aphsp 90和Aphsp 26在介导环境胁迫中起重要作用。- 是的
The heat shock proteins (HSPs) are a family of proteins whose expression is enhanced in response to environmental stressors. TheApostichopus japonicus hsp90andhsp26genes were cloned using expressed sequence tag and rapid amplification of cDNA ends techniques. The full-length cDNA ofAphsp90andAphsp26contains 3,458 and 1,688 nucleotides encoding 720 and 236 amino acids, respectively. Multiple alignments indicated that the deduced amino acid sequences of ApHsp90 and ApHsp26 shared a high level of identity with Hsp90 and small SHPs (sHSPs) sequences of zebrafish, ant, acorn worms, etc., and shared identical structural features with Hsp90 and sHSPs. The expression profiles of these two genes under heat treatment were investigated by real-time quantitative PCR. It was found that the messenger RNA (mRNA) transcripts of the twoA. japonicusgenes varied among different tissues under normal conditions and heat shock, and that the mRNA expression of the two genes was higher in the intestine compared to other tissues. Heat shock significantly elevated the expression ofAphsp90andAphsp26mRNA in a temperature- and time-dependent manner. The results indicate thatAphsp90andAphsp26played important roles in mediating the environmental stress inA. japonicus.