Identification and structure-activity relationship of an antimicrobial peptide of the palustrin-2 family isolated from the skin of the endangered frog Odorrana ishikawae
Identification and structure-activity relationship of an antimicrobial peptide of the palustrin-2 family isolated from the skin of the endangered frog Odorrana ishikawae
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DOI：
10.1016/j.peptides.2011.08.024
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发表时间：
2011-10-01
期刊：
影响因子：
3
通讯作者：
Ukena, Kazuyoshi
中科院分区：
文献类型：
--
作者：
Iwakoshi-Ukena, Eiko;Okada, Genya;Ukena, Kazuyoshi
最近，我们从濒临灭绝的无尾两栖动物Odorrana ishikwae的皮肤中鉴定了九种新的抗菌肽，以评估其天然免疫系统。在这项研究中，根据对大肠杆菌的抗菌活性，初步分离出了一种额外的抗菌肽。该新的抗菌肽属于帕鲁斯特林-2家族，命名为帕鲁斯特林-2ISb。它由36个氨基酸残基组成，其中7个氨基酸位于环七肽Rana盒结构域的C末端。该肽的一级结构表明它与中国臭蛙Odorrana grahami有密切的亲缘关系。克隆的前体蛋白编码基因包含一个信号肽、一个N端酸性间隔区、一个Lys-Arg加工位点和C端前体抗菌肽。在成熟肽的C末端还含有3个氨基酸残基。最后，用合成的几种多肽对四种微生物(大肠杆菌、金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌和白色念珠菌)进行了抑菌活性研究。29个氨基酸的截短形式的多肽，缺少Rana盒的7个氨基酸，具有比天然结构更强的抗菌活性。(C)2011 Elsevier Inc.保留所有权利。
Recently, we identified nine novel antimicrobial peptides from the skin of the endangered anuran species, Odorrana ishikawae, to assess its innate immune system. In this study an additional antimicrobial peptide was initially isolated based on antimicrobial activity against Escherichia coli. The new antimicrobial peptide belonging to the palustrin-2 family was named palustrin-2ISb. It consists of 36 amino acid residues including 7 amino acids C-terminal to the cyclic heptapeptide Rana box domain. The peptide's primary structure suggests a close relationship with the Chinese odorous frog, Odorrana grahami. The cloned cDNA encoding the precursor protein contained a signal peptide, an N-terminal acidic spacer domain, a Lys-Arg processing site and the C-terminal precursor antimicrobial peptide. It also contained 3 amino acid residues at the C-terminus not found in the mature peptide. Finally, the antimicrobial activities against four microorganisms (E. coli, Staphylococcus aureus, methicillin-resistant S. aureus and Candida albicans) were investigated using several synthetic peptides. A 29 amino acid truncated form of the peptide, lacking the 7 amino acids C-terminal to the Rana box, possessed greater antimicrobial activities than the native structure. (C) 2011 Elsevier Inc. All rights reserved.