Structure of the human ubiquitin fusion gene Uba80 (RPS27a) and one of its pseudogenes
Structure of the human ubiquitin fusion gene Uba80 (RPS27a) and one of its pseudogenes
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DOI：
10.1006/bbrc.2000.2568
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发表时间：
2000-04-21
影响因子：
3.1
通讯作者：
Stratakis, CA
中科院分区：
文献类型：
--
作者：
Kirschner, LS;Stratakis, CA
泛素是一种高度保守的76个氨基酸的蛋白质，其在细胞中通过含有多聚泛素链或与羧基延伸蛋白（CEP）融合的泛素的较大蛋白质的蛋白水解产生。在人类中，两个人泛素-CEP基因是Uba 80和Uba 52，它们分别编码与核糖体蛋白S27 a和L40融合的泛素。从最近生成的人类染色体2 p16的物理图谱工作，我们确定了Uba 80基因的遗传和物理位置以及整个基因组结构。Uba 80和Uba 52的比较显示，这两个基因共享保守的5 '端结构，但泛素编码区的结构不保守。400 bp的Uba 80启动子的分析显示，不仅与Uba 52启动子，而且与迄今为止已鉴定的其他已知的人核糖体基因启动子具有很强的相似性。同源性搜索还检测到Uba 80的假基因的存在，并且还报道了该序列特征的结构。(C)北京大学出版社.
Ubiquitin is a highly conserved 76 amino acid protein that is generated in the cell by proteolysis of larger proteins containing either polyubiquitin chains or ubiquitin fused to carboxyl extension proteins (CEPs). In humans, the two human ubiquitin-CEP genes are Uba80 and Uba52, which code for ubiquitin fused to ribosomal protein S27a and L40, respectively, Working from a recently generated physical map of human chromosome 2p16, we determined the genetic and physical location and the genomic structure of the Uba80 gene in its entirety. A comparison of Uba80 to Uba52 revealed that the two genes share a conserved 5'-end structure, but that the structure of the ubiquitin coding regions was not conserved. Analysis of 400 bp of the promoter of Uba80 revealed strong similarity not only to the Uba52 promoter, but also to the other known human ribosomal gene promoters that have been identified to date. Homology searches also detected the presence of a pseudogene for Uba80, and the structure of this sequence feature is also reported. (C) 2000 Academic Press.