LOCALIZATION OF THE MESSENGER-RNA FOR A CHICKEN PRION PROTEIN BY INSITU HYBRIDIZATION
LOCALIZATION OF THE MESSENGER-RNA FOR A CHICKEN PRION PROTEIN BY INSITU HYBRIDIZATION
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DOI：
10.1073/pnas.90.9.4309
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发表时间：
1993-05-01
影响因子：
11.1
通讯作者：
SNIDER, WD
中科院分区：
文献类型：
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作者：
HARRIS, DA;LELE, P;SNIDER, WD
在人类和动物中引起传染性海绵状脑病的感染性病原体(Prion)主要由一种名为PrP(SC)的33-35 kDa的糖蛋白组成，PrP(SC)是正常细胞表面蛋白PrP(C)的翻译后修饰形式。对PrP(C)的功能知之甚少。有趣的是，PrP(C)的鸡肉同系物chPrP与来自大脑的一种刺激骨骼肌细胞上乙酰胆碱受体合成的因子交配。用原位杂交的方法，我们报道了chPrP mRNA广泛分布于成年中枢神经系统的胆碱能和非胆碱能神经元，包括端脑纹、丘脑和下丘脑、视顶盖、延髓、小脑和脊髓。该基因早在胚胎第6天就存在于脑和脊髓中，也存在于背根神经节、视网膜、肠和心脏中。我们的数据表明，如果chPrP起到调节突触后靶点上的乙酰胆碱受体数量的作用，这并不是其唯一的功能。这种蛋白质很可能在中枢神经系统中发挥着更广泛的作用，也许在其他地方也是如此，可能与细胞间的通信、黏附或识别有关。鸡胚胎是研究PrP(C)正常发育功能的一个有吸引力的实验系统。
The infectious agent (prion) responsible for transmissible spongiform encephalopathies in humans and animals is composed primarily of a 33- to 35-kDa glycoprotein called PrP(Sc) (scrapie isoform of prion protein), which is a posttranslationally modified form of the normal cell-surface protein PrP(C). Little is known about the function of PrP(C). Interestingly, chPrP, the chicken homologue of PrP(C), copurifies with a factor from brain that stimulates synthesis of acetylcholine receptors on skeletal muscle cells. Using in situ hybridization, we report here that chPrP mRNA is widely distributed in cholinergic and noncholinergic neurons throughout the adult central nervous system, including those in the telencephalic striata, thalamus and hypothalamus, optic tectum, medulla, cerebellum, and spinal cord. The mRNA is present in the brain and spinal cord as early as embryonic day 6 and is also found in dorsal root ganglia, retina, intestine, and heart. Our data suggest that if chPrP serves to regulate acetylcholine receptor number on postsynaptic targets, this is not its only function. It is likely that the protein plays a more widespread role in the central nervous system and perhaps elsewhere, possibly one related to intercellular communication, adhesion, or recognition. The chicken embryo represents an attractive experimental system in which to investigate the normal developmental function of PrP(C).