Ethanol and diolein stimulate PKC translocation in astroglial cells.
Ethanol and diolein stimulate PKC translocation in astroglial cells.
复制标题
乙醇和二油精刺激星形胶质细胞中的 PKC 易位。
DOI：
10.1016/0024-3205(90)90476-8
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发表时间：
1990
期刊：
影响因子：
6.1
通讯作者：
Shain,W
中科院分区：
文献类型：
--
作者：
Skwish,S;Shain,W
乙醇刺激星形胶质细胞释放牛磺酸。为了确定乙醇是否使用蛋白激酶C（PKC）介导这种释放，使用LRM 55星形胶质细胞测量PKC活性。当将乙醇（25-200 mM）或二油（3 μM）应用于细胞30秒时，观察到细胞质中的PKC活性降低，细胞膜部分增加，而整个细胞活性保持不变。膜相关活性几乎增加了100%。当同时应用乙醇（100 mM）和二油精（3 μM）时，膜相关活性增加，比单独应用任何一种PKC激活剂时大3-5倍。当细胞暴露于乙醇和PKC激活剂二油酸甘油酯时，观察到PKC活性的这些变化与牛磺酸释放的变化平行。乙醇刺激的释放可能与PKC活性从胞浆到膜的易位有关。
Ethanol exposure stimulates taurine release from astroglial cells. To determine if ethanol mediates this release using protein kinase C (PKC), PKC activity was measured using LRM55 astroglial cells. When ethanol (25–200 mM) or diolei (3 μM) was applied to cells for 30 seconds, PKC activity was observed to decrease in the cytosol and increase in the membrane fraction of the cell while the whole cell activity remained unchanged. The membrane-associated activity increased by almost 100%. When ethanol (100 mM) and diolein (3 μM) were applied simultaneously, membrane-associated activity increased to become 3–5 times greater than when either PKC activator was applied alone. These changes in PKC activity parallel changes in taurine release observed when cells are exposed to ethanol and the PKC activator diolein. Ethanol-stimulated release may be associated with the translocation of PKC activity from the cytosol to the membrane.