Growth of normal and malignant human mammary epithelial cells in culture.
Growth of normal and malignant human mammary epithelial cells in culture.
复制标题
正常和恶性人类乳腺上皮细胞在培养物中的生长。
DOI：
10.1093/jnci/63.1.29
复制
发表时间：
1979
期刊：
影响因子：
--
通讯作者：
P. Furmanski
中科院分区：
文献类型：
--
作者：
W. Kirkland;N. Yang;T. Jorgensen;C. Longley;P. Furmanski
将正常和恶性人乳腺上皮细胞置于培养物中。正常细胞从泌乳后期的乳汁和乳腺液中回收，恶性细胞通过胶原酶消化从原发性乳腺肿瘤中分离。从乳腺液样品中获得的细胞浓度与分泌的液体体积成反比。这些细胞中的大多数快速粘附于基质，不复制，显示Fc受体依赖性吞噬活性，因此被鉴定为巨噬细胞。剩余的细胞生长成由一种或两种不同形态类型的乳腺上皮细胞组成的大岛。这些细胞的最佳生长在缓冲至pH 6.8的培养基中获得，表皮生长因子显着延长细胞的指数生长期。两个形态学上不同的上皮细胞群体也观察到从个别乳腺肿瘤建立的文化。恶性细胞的生长相对不受培养基pH的影响，并且细胞对外源性添加的激素无反应。基质成纤维细胞在原代培养中的恶性上皮细胞的过度生长被延迟，用含有血清替代品的新鲜培养基替换标准生长培养基;恶性上皮细胞的生长不受影响。丝裂霉素C处理的人成纤维细胞的饲养层增加了正常和恶性细胞在原代培养中的铺板效率，并且还促进了这些细胞向第二和第三培养物的传代。
Normal and malignant human mammary epithelial cells were placed in culture. Normal cells were recovered from late-lactation milk and breast fluids, and malignant cells were isolated from primary breast tumors by collagenase digestion. The concentration of cells obtained from breast fluid samples was inversely proportional to the volume of fluid secreted. Most of these cells adhered rapidly to the substrate, did not replicate, displayed Fc receptor-dependent phagocytic activity, and were thus identified as macrophages. The remaining cells grew out into large islands comprised of one or two distinct morphologic types of mammary epithelial cells. Optimum growth of these cells was obtained in medium buffered to pH 6.8, and the epidermal growth factor markedly prolonged the exponential growth phase of the cells. Two morphologically distinct populations of epithelial cells were also observed in cultures established from individual breast tumors. Growth of the malignant cells was relatively unaffected by the pH of the culture medium, and the cells were unresponsive to exogenously added hormones. Overgrowth of malignant epithelial cells in primary cultures by stromal fibroblasts was retarded by replacement of standard growth medium with fresh medium containing a serum substitute; growth of the malignant epithelial cells was unaffected. A feeder layer of mitomycin C-treated human fibroblasts increased the plating efficiency of both normal and malignant cells in primary culture and also facilitated passage of these cells to secondary and tertiary cultures.