ben Functions with scamp during synaptic transmission and long-term memory formation in Drosophila.
ben Functions with scamp during synaptic transmission and long-term memory formation in Drosophila.
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DOI：
10.1523/jneurosci.5036-07.2009
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发表时间：
2009-01-14
期刊：
影响因子：
--
通讯作者：
Tully T
中科院分区：
文献类型：
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作者：
Zhao H;Zheng X;Yuan X;Wang L;Wang X;Zhong Y;Xie Z;Tully T
对有巴甫洛夫嗅觉记忆缺陷的果蝇突变体的基因筛选为记忆储存的分子基础提供了独特的见解。偶尔，这些单一的遗传病变被组装成有意义的分子通路和神经回路。然而，在大多数情况下，这些基因及其在中枢神经系统中的表达模式仍然是碎片化的，需要从持续的突变筛选中获得新的线索。从长期记忆（LTM）突变体的行为筛选中，我们发现了ben (CG32594)，它编码一种新的蛋白质。ben的突变特别破坏了LTM，使早期的记忆阶段保持完整。ben的作用似乎是生理上的，而不是发育上的，因为在成人中急性诱导ben+转基因的表达可以挽救突变体的LTM缺陷。更有趣的是，诱导ben+特异性表达在蘑菇体（mb）中，而不是在中心复合体的椭球体中，足以挽救突变体LTM缺陷。这表明ben在嗅觉记忆形成过程中参与了MB的作用。我们还提供证据表明，BEN在突触传递和LTM形成中与SCAMP（一种已知参与囊泡循环的突触蛋白）发生遗传相互作用。
Genetic screens for Drosophila mutants defective in pavlovian olfactory memory have provided unique insight into the molecular basis of memory storage. Occasionally, these singular genetic lesions have been assembled into meaningful molecular pathways and neural circuitries. For the most part, however, these genes and their expression patterns in the CNS remain fragmented, demanding new clues from continued mutant screens. From a behavioral screen for long-term memory (LTM) mutants, we have identified ben (CG32594), which encodes a novel protein. Mutations of ben specifically disrupt LTM, leaving earlier memory phases intact. The role of ben appears physiological rather than developmental, because acutely induced expression of a ben+ transgene in adults rescues the mutant’s LTM defect. More interestingly, induced expression of ben+ specifically in mushroom bodies (MBs), but not in the ellipsoid body of the central complex, is sufficient to rescue the mutant LTM defect. This suggests a role for ben in the MB during olfactory memory formation. We also provide evidence that BEN interacts genetically in both synaptic transmission and LTM formation with SCAMP, a synaptic protein known to be involved in vesicle recycling.