Placenta growth factor and vascular endothelial growth factor a have differential, cell-type specific patterns of expression in vascular cells.
Placenta growth factor and vascular endothelial growth factor a have differential, cell-type specific patterns of expression in vascular cells.
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胎盘生长因子和血管内皮生长因子 a 在血管细胞中具有不同的、细胞类型特异性的表达模式。
DOI：
10.1111/micc.12113
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Lloyd,PamelaG
中科院分区：
文献类型：
--
作者：
Xiang,Lingjin;Varshney,Rohan;Rashdan,NabilA;Shaw,JenniferH;Lloyd,PamelaG
目的血管内皮生长因子(PLGF)是一种血管内皮生长因子-A相关蛋白，通过单核细胞介导侧支扩张，但在毛细血管增殖中作用不大。相反，血管内皮生长因子-A同时调节侧支循环增大和毛细血管增殖。与血管内皮生长因子A相比，对PlGF的研究还不够深入，关于其调控和功能的问题仍然存在。因此，我们的目标是研究PLGF在血管细胞中的表达。我们假设血管SMC比EC表达更多的PLGF，因为VEGF-A主要由非EC表达。方法比较8株EC和SMC的PLGF和VEGF-A的表达，然后下调PLGF并评价细胞功能。结果PLGF在EC中高表达，而VEGF-A在SMC中高表达。PlGF基因敲除不影响EC的数量、迁移或管状形成，但减少了单核细胞向EC的迁移。外源性PLGF可挽救单核细胞迁移。缺氧使PLGF蛋白表达增加，但不激活PLGF基因转录。结论PLGF和VEGF-A在血管细胞中有不同的表达模式。EC来源的PLGF可能主要在EC和循环细胞之间的通讯中起作用。低氧可在转录后增加EC PLGF的表达。
ObjectivePLGF, a VEGF‐A related protein, mediates collateral enlargement via monocytes but plays little role in capillary proliferation. In contrast, VEGF‐A mediates both collateral enlargement and capillary proliferation. PLGF has been less thoroughly studied than VEGF‐A, and questions remain regarding its regulation and function. Therefore, our goal was to characterize the expression of PLGF by vascular cells. We hypothesized that vascular SMC would express more PLGF than EC, since VEGF‐A is primarily expressed by non‐EC.MethodsWe compared PLGF and VEGF‐A across eight EC and SMC lines, then knocked down PLGF and evaluated cell function. We also assessed the effect of hypoxia on PLGF expression and promoter activity.ResultsPLGF was most highly expressed in EC, whereas VEGF‐A was most highly expressed in SMC. PLGF knockdown did not affect EC number, migration, or tube formation, but reduced monocyte migration toward EC. Monocyte migration was rescued by exogenous PLGF. Hypoxia increased PLGF protein without activating PLGF gene transcription.ConclusionsPLGF and VEGF‐A have distinct patterns of expression in vascular cells. EC derived PLGF may function primarily in communication between EC and circulating cells. Hypoxia increases EC PLGF expression posttranscriptionally.