The four biochemically distinct species of human interleukin 1 all exhibit similar biologic activities.
The four biochemically distinct species of human interleukin 1 all exhibit similar biologic activities.
复制标题
人类白细胞介素 1 的四种生化特性不同的物种都表现出相似的生物活性。
作者：
D. Wood;E. Bayne;M. Goldring;M. Gowen;D. Hamerman;J. Humes;E. J. Ihrie;P. Lipsky;M. Staruch
人单核细胞与内毒素孵育的上清液在PHA存在下能刺激小鼠胸腺细胞增殖。这被称为LAF（淋巴细胞活化因子）活性，是白细胞介素1（IL 1）的特征活性。LAF活性可分为四个主要部分：15，000道尔顿（pI 7）、15，000（pI 5.5）、35，000（pI 7）和35，000（pI 5.5）部分。为了确定这四种组分是否具有归因于IL 1的其他生物活性，在一系列生物测定中对它们进行比较。当相对于它们的LAF活性标准化时，这四种组分作为鼠胸腺细胞的有丝分裂原、IL 2的诱导剂、鼠或人B细胞激活剂、人软骨细胞或滑膜细胞刺激剂或体内急性期蛋白的诱导剂没有显著差异。另一方面，样品作为猪滑膜细胞的刺激物明显不同，15，000道尔顿（pI 5.5）级分是唯一的强活性级分。这些结果与所有四种LAF可能是单个基因的产物的假设一致，尽管猪受体可能能够区分它们。如果是这样的话，所有四个部分都可以适当地称为IL 1。
The supernatants of human monocytes incubated with endotoxin are able to stimulate the proliferation of murine thymocytes in the presence of PHA. This is known as LAF (lymphocyte activating factor) activity and is a characteristic activity of interleukin 1 (IL 1). The LAF activity can be resolved into four major fractions: a 15,000 dalton (pI 7), a 15,000 (pI 5.5), a 35,000 (pI 7), and a 35,000 (pI 5.5) fraction. To determine whether these four fractions shared the other biologic activities ascribed to IL 1, they were compared in a series of bioassays. When standardized with respect to their LAF activities, the four fractions did not differ significantly as mitogens for murine thymocytes, inducers of IL 2, murine or human B cell activators, human chondrocyte or synoviocyte stimulants, or inducers of acute phase proteins in vivo. On the other hand, the samples differed markedly as stimulators of porcine synoviocytes, with the 15,000 dalton (pI 5.5) fraction being the only strongly active fraction. These results are consistent with the hypothesis that all four LAF could be products of a single gene, although the porcine receptor may be able to distinguish between them. If this is the case, all four fractions can properly be termed IL 1.