[A new liver support system composed of functional human cells and a radial-flow bioreactor].
[A new liver support system composed of functional human cells and a radial-flow bioreactor].
复制标题
[一种由功能性人体细胞和径向流生物反应器组成的新型肝脏支持系统]。
作者：
M. Kawada;S. Nagamori;H. Aizaki;K. Fukaya;M. Niiya;T. Matsuura;H. Sujino;S. Hasumura;K. Shimizu;H. Yoshida
人工肝是治疗急性肝功能衰竭的有效手段，也是肝移植的桥梁。目前的报道表明，由功能性人肝细胞和生物反应器组成的混合型人工肝是实用的临床应用。在本研究中，我们成功地在大规模高密度培养人功能性肝癌（JHH-7）使用新开发的径向流填充床生物反应器。由于该生物反应器的切应力低于其他类型，因此可以进行无细胞损伤的高密度培养。JHH-7细胞能产生大量的人血白蛋白等肝脏特异性蛋白，在系统中具有氨代谢功能。径向流生物反应器有望成为一种新型的人工肝。
An artificial liver will be useful for the treatment of acute hepatic failure and a bridge of liver transplantation. The current reports suggest that the hybrid type of artificial liver composed of functional human liver cells and a bioreactor is practical for clinical use. In the present study, we succeeded high density culture on a large-scale of human functional hepatoma (JHH-7) using a newly developed radial flow packed-bed bioreactor. Since the shear stress of this bioreactor is lower than the other type, high density culture without cell damage is possible. JHH-7 cells produced large amounts of human albumin and other liver specific proteins, and then have the function of ammonia metabolism in the system. This study suggests that a radial flow bioreactor will be developed as a new type of artificial liver.