Long noncoding RNA SPRY4-IT1 is upregulated in esophageal squamous cell carcinoma and associated with poor prognosis
Long noncoding RNA SPRY4-IT1 is upregulated in esophageal squamous cell carcinoma and associated with poor prognosis
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长非编码RNA SPRY4-IT1在食管鳞状细胞癌中表达上调并与不良预后相关
DOI：
10.1007/s13277-014-2013-y
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发表时间：
2014-08-01
期刊：
影响因子：
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通讯作者：
Cao, Xiu-Feng
中科院分区：
文献类型：
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作者：
Xie, Hai-Wei;Wu, Qing-Quan;Cao, Xiu-Feng
LncRNA SPRY4-IT1已被证明可促进黑色素瘤的进展。然而，lncRNA SPRY4-IT1在人食管鳞状细胞癌（ESCC）中的作用尚不清楚。本研究旨在探讨SPRY4-IT1在ESCC中的临床意义及生物学功能。采用定量逆转录聚合酶链式反应（qRT-PCR）技术检测92例ESCC患者和8株ESCC细胞株中lncRNA SPRY4-IT的表达水平。采用Kaplan-Meier和Cox回归分析评估预后意义。小干扰RNA （Small interfering RNA, siRNA）抑制SPRY4-IT1在ESCC细胞系中的表达。体外和体内实验进一步探讨其在肿瘤进展中的作用。SPRY4-IT1在ESCC组织和细胞中的表达水平明显高于相应的癌旁非癌组织和非致瘤性食管上皮细胞，SPRY4-IT1高表达的ESCC患者比SPRY4-IT1低表达的ESCC患者临床分期晚，预后差。多因素分析显示SPRY4-IT1表达水平是ESCC患者的独立预后因素。体外实验表明，SPRY4-IT1的敲低可减少细胞增殖、侵袭性和迁移。体内实验表明，SPRY4-IT1的下调会降低细胞生长。SPRY4-IT1是参与ESCC进展的新分子，可能提供潜在的预后生物标志物和治疗干预的潜在靶点。
LncRNA SPRY4-IT1 has been shown to promote the progression of melanoma. However, the role of lncRNA SPRY4-IT1 in human esophageal squamous cell carcinoma (ESCC) remains unclear. The purpose of this study is to investigate the clinical significance and biological functions of SPRY4-IT1 in ESCC. The expression levels of lncRNA SPRY4-IT in 92 ESCC patients and 8 ESCC cell lines were evaluated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The prognostic significance was evaluated using Kaplan–Meier and Cox regression analyses. Small interfering RNA (siRNA) was used to suppress SPRY4-IT1 expression in ESCC cell lines. Both in vitro and in vivo assays were performed to further explore its role in tumor progression. SPRY4-IT1 levels were significantly higher in ESCC tissues and cells than in corresponding adjacent noncancerous tissues and nontumorigenic esophageal epithelial cells, and the ESCC patients with higher SPRY4-IT1 expression had an advanced clinical stage and poorer prognosis than those with lower SPRY4-IT1 expression. The multivariate analysis revealed that SPRY4-IT1 expression level is an independent prognostic factor in ESCC patients. In vitro assays demonstrated that knockdown of SPRY4-IT1 reduced cell proliferation, invasiveness, and migration. In vivo assays demonstrated that knockdown of SPRY4-IT1 decreases cell growth. SPRY4-IT1 is a novel molecule involved in ESCC progression, which may provide a potential prognostic biomarker and a potential target for therapeutic intervention.