Up-regulated expression of Notch1 and Jagged1 in human colon adenocarcinoma
Up-regulated expression of Notch1 and Jagged1 in human colon adenocarcinoma
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DOI：
10.1016/j.patbio.2010.11.001
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发表时间：
2011-12-01
影响因子：
--
通讯作者：
Zheng, G.
中科院分区：
文献类型：
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作者：
Gao, J.;Liu, J.;Zheng, G.
Notch分子在许多恶性肿瘤中表达失调，但Notch 1和Jagged 1在结肠腺癌中的表达尚不清楚。本研究旨在探讨Notch 1和Jagged 1在人结肠腺癌中的表达。应用免疫组化方法检测65例结肠腺癌和60例癌旁正常结肠组织切片中的表达。对10对新鲜手术切除的人结肠腺癌和邻近非肿瘤结肠标本进行Western blot和RT-PCR分析。Notch 1和Jagged 1均表达于结肠腺癌组织中的肿瘤细胞胞浆中。这两种分子的蛋白和mRNA水平在结肠腺癌中均高于癌旁组织。Notch 1与肿瘤分期呈正相关。这项研究表明，Notch 1和Jagged 1在人类结肠腺癌中上调，并表明Notch 1/Jagged 1信号转导可能在结肠腺癌的发展中发挥作用。(C)2010年Elsevier Masson SAS。All rights reserved.
Deregulated expression of Notch molecules is observed in many malignant tumors, however, the expression of Notch1 and Jagged1 in colon adenocarcinoma is still unknown. This study is to investigate the expression of Notch1 and Jagged1 in human colon adenocarcinoma. Sixty-five human colon adenocarcinoma and 60 adjacent nontumor colon tissue sections were detected by immunohistochemistry. Ten paired fresh surgical human colon adenocarcinoma and adjacent nontumor colon samples were analyzed by Western blot and RT-PCR. Both Notch1 and Jagged1 were expressed in the cytoplasm of neoplastic cells of colon adenocarcinoma tissue. The protein and mRNA levels of both molecules were higher in colon adenocarcinoma than in adjacent nontumor tissue. Moreover, Notch1 was positively correlated with tumor stage. This investigation demonstrates that Notch1 and jagged1 are up-regulated in human colon adenocarcinoma and suggests that Notch1/Jagged1 signaling might play a role in the development of colon adenocarcinoma. (C) 2010 Elsevier Masson SAS. All rights reserved.