Biosynthesized silver nanoparticles using Bacillus amyloliquefaciens; Application for cytotoxicity effect on A549 cell line and photocatalytic degradation of p-nitrophenol
Biosynthesized silver nanoparticles using Bacillus amyloliquefaciens; Application for cytotoxicity effect on A549 cell line and photocatalytic degradation of p-nitrophenol
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DOI：
10.1016/j.jphotobiol.2019.111642
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发表时间：
2020-01-01
影响因子：
5.4
通讯作者：
Pugazhendhi, Arivalagan
中科院分区：
文献类型：
--
作者：
Samuel, Melvin S.;Jose, Sujin;Pugazhendhi, Arivalagan
本研究报道了利用淀粉解聚芽孢杆菌MSR5生物合成银纳米颗粒。淀粉菌的无细胞培养上清液对AgNPs的合成起到了稳定剂的作用。利用紫外可见分光光度计、X射线衍射仪、傅立叶变换红外光谱、扫描电子显微镜、差示扫描量热仪和透射电子显微镜对所合成的AgNPs进行了表征。透射电子显微镜图像显示生物合成的AgNPs呈球形，范围为20-40 nm。本研究采用超声辐照法制备了AgNPs。用Zeta电位法测得AgNPs的稳定性为-33.4 mV。在太阳光照射下，考察了AgNPs对4-硝基苯酚(4-NP)的催化降解，并研究了几种降解参数对降解效果的影响。生物合成的AgNPs具有很强的化学催化作用，NaBH4在15min内可将4-NP(98%)综合降解为4-氨基酚(4-AP)。此外，采用四甲基偶氮唑盐比色法测定了10~200mgAgNPs的细胞毒性。结果表明，AgNPs对A549细胞有明显的抑制作用，且呈剂量依赖性。研究表明，利用无细胞培养上清液合成的AgNPs可用于去除废水中的有害污染物。
The present study reports the biosynthesis of silver nanoparticles (AgNPs) using Bacillus amyloliquefaciens MSR5. The cellfree supernatant of B. amyloliquefaciens acted as a stabilizing agent for the synthesis of AgNPs. The synthesized AgNPs were characterized using UV-vis spectrophotometer, PXRD, FTIR, SEM-EDX, DLS, and TEM. TEM image showed the spherical shape of the biosynthesized AgNPs and it was found to be 20-40 nm in range. In this study, the AgNPs were prepared by ultrasonic irradiation. The stability of the AgNPs was found to be -33.4 mV using zeta potential. The catalytic 4-nitrophenol (4-NP) degradation by AgNPs was examined under solar irradiation and furthermore, the effects of several degradation parameters were studied. The biosynthesized AgNPs exhibited a strong chemocatalytic action with a comprehensive degradation (98%) of 4-NP to 4-aminophenol (4-AP) using NaBH4 within 15 min. In addition, MTT assay was performed to evaluate the cytotoxicity of the biosynthesized AgNPs (10 - 200 mu g). The results have shown that the AgNPs exhibited significant activity on A549 cells, which was dosedependent. The study elucidates the AgNPs synthesized using cellfree culture supernatant can be used for the elimination of hazardous pollutants from wastewater.