A new application of a commercial microtiter plate-based assay for assessing the antimicrobial activity of Maillard reaction products
A new application of a commercial microtiter plate-based assay for assessing the antimicrobial activity of Maillard reaction products
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DOI：
10.1016/j.foodres.2005.06.002
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发表时间：
2006
影响因子：
8.1
通讯作者：
J. Rufián‐Henares;F. Morales
中科院分区：
文献类型：
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作者：
J. Rufián‐Henares;F. Morales
一种新的应用程序的商业微量滴定板为基础的测定开发的定量筛选过程中形成的食品热处理的抗菌化合物。这种被称为美拉德反应产物（MRP）的化合物广泛分布于西方国家的饮食中。报道的方法是快速，廉价和容易的，有利于剂量-反应曲线的生成，允许计算大多数物质的抗微生物活性的同时作为最低抑菌浓度（MIC）或作为土霉素当量值（OTEV）。该检测准确且重现性高（日间和日间变异分别为2.3%和1.8%）。对于测试的样品，在咖啡类黑精（MRP的高分子量部分）中发现了较高的抗微生物活性，尽管非共价连接的类黑精连接的化合物也显示出抗微生物活性。此外，来自更严重处理的样品的类黑精表现出更高的抑制细菌生长活性，例如CTn 60咖啡（最高烘焙程度）和黑啤酒。
A new application of a commercial microtiter plate-based assay was developed for the quantitative screening of antimicrobial compounds formed during the thermal treatment of foods. Such compounds called Maillard reaction products (MRP) are widely distributed in the diet of western countries. The reported method is fast, cheap and easy and facilitates the generation of a dose–response curve which allows calculating the antimicrobial activity of most substances at the same time as minimum inhibitory concentration (MIC) or as oxytetracyclin equivalent value (OTEV). The test is accurate and highly reproducible (inter- and intra-day variation of 2.3% and 1.8%, respectively). For the tested samples, the higher antimicrobial activity was found in coffee melanoidins (high molecular weight fraction of MRP) although non-covalently melanoidins-linked compounds showed antimicrobial activity too. In addition, melanoidins from more severely treated samples exerted higher inhibitory bacterial growing activity, such as CTn60 coffee (highest roasting degree) and dark beer.