Rapid separation and identification of multiple constituents in traditional Chinese medicine formula Shenqi Fuzheng Injection by ultra-fast liquid chromatography combined with quadrupole-time-of-flight mass spectrometry
Rapid separation and identification of multiple constituents in traditional Chinese medicine formula Shenqi Fuzheng Injection by ultra-fast liquid chromatography combined with quadrupole-time-of-flight mass spectrometry
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DOI：
10.1016/j.jpba.2012.10.024
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发表时间：
2013-02-23
影响因子：
3.4
通讯作者：
Su, Wei-Wei
中科院分区：
文献类型：
--
作者：
Liu, Meng-Hua;Tong, Xin;Su, Wei-Wei
参七扶正注射液(SFI)是一种著名的中药方剂，在临床上被广泛用于癌症化疗的辅助治疗。然而，到目前为止，对SFI的化学成分，特别是水溶性成分的研究还不多见。本研究建立了超快速液相色谱(UFLC)与电喷雾电离四极杆飞行时间质谱仪(ESI-Q-TOF-MS/MS)联用的超快速分离和结构鉴定方法。色谱柱为C18反相柱(2.1 mm×100 mm，1.8µm)，流动相为含0.1%甲酸的甲醇-水，流速为0.2mL/min。对分子离子和特征碎片离子的准确质量测量可以为这些化合物提供可靠的鉴定标准。通过保留时间和MS谱图与标准或文献数据的比较，鉴定或初步鉴定了81个主要成分，包括有机酸、氨基酸、低聚糖、生物碱、核苷、苯丙烷类、聚乙炔、黄酮类、异黄酮类和皂苷。所有化合物都在单独的原料中进一步进行了分配。总之，UFLC-Q-TOF-MS/MS是一种高效分离和鉴定中药复杂基质中化学成分的技术。本研究结果为SFI的质量控制和体内进一步研究提供了依据。皇冠版权所有(C)2012由爱思唯尔出版。保留所有权利。
Shenqi Fuzheng Injection (SFI) a well-known traditional Chinese medicine (TCM) formula, has been extensively used as an adjuvant to chemotherapy for cancer treatment in clinic. However, the chemical constituents in SFI, especially water-soluble ingredients, had not been investigated so far. In this study, an ultra-fast liquid chromatography (UFLC) coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS/MS) method was established for rapid separation and structural identification of the constituents in SFI. Separation was performed on a C18 reversed-phase column (2.1 mm x 100 mm, 1.8 mu m) by gradient elution mode, using methanol-water containing 0.1% formic acid as mobile phase at the flow-rate of 0.2 mL/min. Accurate mass measurement for molecular ions and characteristic fragment ions could represent reliable identification criteria for these compounds. As a result, eighty-one major constituents including organic acids, amino acids, oligosaccharides, alkaloids, nucleosides, phenylpropanoids, polyacetylenes, flavonoids, isoflavonoids and saponins were identified or tentatively characterized by comparing their retention times and MS spectra with those of authentic standards or literature data. All compounds were further assigned in the individual raw material. In conclusion, the UFLC-Q-TOF-MS/MS is a highly efficient technique to separate and identify constituents in complex matrices of traditional Chinese medicines. These results obtained in this research will provide a basis for quality control and further study in vivo of SFI. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.