Antimicrobial Resistance and Virulence Genes Distribution in Trueperella pyogenes Isolated from Dairy Cows with Clinical Mastitis in Liaoning of China
Antimicrobial Resistance and Virulence Genes Distribution in Trueperella pyogenes Isolated from Dairy Cows with Clinical Mastitis in Liaoning of China
复制标题
辽宁临床乳腺炎奶牛化脓性Trueperella的耐药性及毒力基因分布
DOI：
10.29261/pakvetj/2021.040
复制
发表时间：
2021-01-01
影响因子：
2.3
通讯作者：
Zhang, Dexian
中科院分区：
文献类型：
--
作者：
Guo, Yuru;Qiu, Peng;Zhang, Dexian
化脓性杜氏杆菌是引起乳腺炎、乳腺炎、肺炎、肝衰竭等多种疾病的病原菌。T.化脓性链球菌可表达plo、fimA、cbpA、nanH和nanP等毒力基因，这些毒力基因有助于其致病性。本研究的目的是调查该菌的耐药性、毒力基因的分布和毒力基因表达盒。化脓性链球菌分离自患有临床型乳房炎的奶牛。采用微量肉汤稀释法测定细菌对不同抗菌药物的敏感性，聚合酶链反应（PCR）检测细菌毒力基因和基因盒。在162头患乳腺炎的奶牛中，10.49%（17/162）的乳样检出T。化脓对克林霉素（23.53%）、土霉素（23.53%）、环丙沙星（47.06%）、磺胺甲恶唑/甲氧苄啶（100%）的耐药率较高。所有分离株均携带Ⅰ类整合子，基因盒阵列为aadA 9（2/17）或aadA 5-dfrA 17（3/17）。所有菌株均携带plo nanH和fimA基因，但编码其他毒力基因的基因（fimC、fimE、nanP和cbpA）的比例在47.06%~ 88.23%之间。我们的研究表明T.从患有临床型乳房炎的奶牛中分离的化脓菌对β-内酰胺类药物敏感。此外，7个毒力基因均存在于分离株中，其中plo、nanH和fimA基因在T.中国辽宁省的化脓菌。
Trueperella pyogenes is considered as a causative agent of many infections, such as mastitis, endometritis, pneumonia, liver abscessation. T. pyogenes can express several virulence genes such as plo, fimA, cbpA, nanH and nanP contributing to its pathogenicity. The aim of this study was to provide an investigation about antimicrobial resistance, as well as virulence genes distribution and gene cassettes among T. pyogenes isolates from dairy cows with clinical mastitis. The susceptibility to different antimicrobial agents was determined by the Broth Microdilution Method, and virulence genes and gene cassette was detected by polymerase chain reactions (PCRs). There are 10.49% (17/162) of milk samples from dairy cows with mastitis were positive for T. pyogenes. High levels of resistance were found to clindamycin (23.53%), oxytetracycline (23.53%), ciprofloxacin (47.06%), sulfamethoxazole/trimethoprim (100%). Moreover, all isolates carried class I integrons, and gene cassette arrays were aadA9 (2/17) or aadA5-dfrA17 (3/17). Finally, all isolates harbored plo nanH and fimA genes, but other genes encoding virulence genes including fimC, fimE, nanP and cbpA are ranged from 47.06% to 88.23%. Our study showed T. pyogenes isolates from dairy cows with clinical mastitis were susceptible to beta-lactams. In addition, all seven virulence genes occurred in isolates, and plo, nanH, and fimA gene showed a significantly higher frequency in T. pyogenes of the Liaoning Province, China.