Molecular architecture of the inner membrane of mitochondria from rat liver: a combined biochemical and stereological study.
Molecular architecture of the inner membrane of mitochondria from rat liver: a combined biochemical and stereological study.
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DOI：
10.1083/jcb.102.1.97
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发表时间：
1986-01
期刊：
影响因子：
--
通讯作者：
Weibel ER
中科院分区：
文献类型：
--
作者：
Schwerzmann K;Cruz-Orive LM;Eggman R;Sänger A;Weibel ER
采用体视学和生物化学相结合的方法研究了线粒体及其内膜的分子结构。通过体视学方法估计来自大鼠肝脏的线粒体纯化制剂中线粒体结构的数量（体积、数量、内膜表面积）。在同一制剂中，通过生化方法测量不同底物的呼吸链的氧化活性和氧化还原复合物的浓度。通过体视学和生化数据的关联，估计从大鼠肝脏中分离出的单个线粒体的体积为0.27微米3，内膜面积为6.5微米2，并含有2,600个（复合物I）至15,600个（aa3）氧化还原复合物，以丙酮酸为底物，每秒产生超过100,000个电子的电子流。各个氧化还原复合物和 H+-ATP 酶一起以大约 7,500/微米 2 的密度出现，并占据大约 40% 的内膜面积。从各自的密度可以得出结论，反应伙伴之间的平均最近距离足够小（70-200 A），足以导致微聚集体的形成。讨论了这些结果对于线粒体能量转导机制的意义。
The molecular structure of mitochondria and their inner membrane has been studied using a combined approach of stereology and biochemistry. The amount of mitochondrial structures (volume, number, surface area of inner membrane) in a purified preparation of mitochondria from rat liver was estimated by stereological procedures. In the same preparation, the oxidative activity of the respiratory chain with different substrates and the concentration of the redox complexes were measured by biochemical means. By relating the stereological and biochemical data, it was estimated that the individual mitochondrion isolated from rat liver has a volume of 0.27 micron 3, an inner membrane area of 6.5 micron 2, and contains between 2,600 (complex I) and 15,600 (aa3) redox complexes which produce an electron flow of over 100,000 electrons per second with pyruvate as substrate. The individual redox complexes and the H+-ATPase together occur at a density of approximately 7,500/micron 2 and occupy approximately 40% of the inner membrane area. From the respective densities it was concluded that the mean nearest distance between reaction partners is small enough (70-200 A) to cause the formation of micro-aggregates. The meaning of these results for the mechanism of mitochondrial energy transduction is discussed.