Reciprocal connectivity between secondary auditory cortical field and amygdala in mice.
Reciprocal connectivity between secondary auditory cortical field and amygdala in mice.
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小鼠次级听觉皮层和杏仁核之间的相互连接。
DOI：
10.1038/s41598-019-56092-9
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Shibuki K
中科院分区：
文献类型：
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作者：
Tsukano H;Hou X;Horie M;Kitaura H;Nishio N;Hishida R;Takahashi K;Kakita A;Takebayashi H;Sugiyama S;Shibuki K
最近的研究已经检查了从杏仁核到听觉皮层的反馈通路，以及从听觉皮层到杏仁核的前馈通路。然而，这些联系尚未得到充分的表征。在这里，可视化的听觉皮层和杏仁核之间的全面连接，我们注射霍乱毒素亚基B（CT B），双向示踪剂，到多个子域在小鼠听觉皮层后，确定这些子域的位置，使用黄素蛋白荧光成像。将CTB注入次级听野（A2）后，发现CTB阳性轴突终末主要分布于外侧杏仁核（La），基底杏仁核等其他部位也有少量分布。此外，我们发现大量的逆行染色CTB阳性神经元后，注入A2的La。当将CTB注射到初级听觉皮层（A1）时，在杏仁核中可见少量CTB阳性神经元和轴突。最后，我们发现其他听觉皮层区域和杏仁核之间几乎完全没有联系。这些数据表明，A2和La之间的相互联系是小鼠听觉皮层和杏仁核之间的主要沟通渠道。
Recent studies have examined the feedback pathway from the amygdala to the auditory cortex in conjunction with the feedforward pathway from the auditory cortex to the amygdala. However, these connections have not been fully characterized. Here, to visualize the comprehensive connectivity between the auditory cortex and amygdala, we injected cholera toxin subunit b (CTB), a bidirectional tracer, into multiple subfields in the mouse auditory cortex after identifying the location of these subfields using flavoprotein fluorescence imaging. After injecting CTB into the secondary auditory field (A2), we found densely innervated CTB-positive axon terminals that were mainly located in the lateral amygdala (La), and slight innervations in other divisions such as the basal amygdala. Moreover, we found a large number of retrogradely-stained CTB-positive neurons in La after injecting CTB into A2. When injecting CTB into the primary auditory cortex (A1), a small number of CTB-positive neurons and axons were visualized in the amygdala. Finally, we found a near complete absence of connections between the other auditory cortical fields and the amygdala. These data suggest that reciprocal connections between A2 and La are main conduits for communication between the auditory cortex and amygdala in mice.