Interaction of Lolium perenne and Hyphomicrobium sp. GHH enhances the removal of 17-ethinyestradiol (EE2) from soil
Interaction of Lolium perenne and Hyphomicrobium sp. GHH enhances the removal of 17-ethinyestradiol (EE2) from soil
复制标题
多年生黑麦草和丝菌属 sp. 的相互作用。
DOI：
10.1007/s11368-018-2116-y
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发表时间：
2019-03-01
影响因子：
3.6
通讯作者：
Lu, Li
中科院分区：
文献类型：
--
作者：
He, Shanying;Guo, Haihui;Lu, Li
目的是增加污水污泥和动物粪便的土地应用已加速了17-乙基雌二醇（EE2）的投入到土壤和环境中，这将影响蔬菜的健康以及动物的荷尔蒙体系。为了从土壤中去除EE2，一种可能的选择是使用EE2降解的微生物或/和/和EE2蓄积植物进行生物修复。该研究试图将EE2降解的微生物从土壤中分离出来，并研究这种微生物和/或有机污染物可去除的植物Lolium Perenne对从土壤中去除EE2的作用。材料和Methodee2ecee2-degradegradegradegradegradectradegradecteria secteria septeria spermicrobobim sp。 GHH是从农田获得的。进行了盆栽实验，以研究接种菌粒sp。 GHH，L。Perenne的培养和菌丝体的接种。 GHH +在EE2从土壤中去除EE2上的L. Perenne的培养。确定植物生物量，植物根和芽中的EE2浓度，植物修复效率以及土壤尿素酶活性和脱氢酶活性，以评估植物和微生物在土壤中去除EE2中的相互作用。从土壤中去除EE2。 GHH显着增加了根和芽中佩伦的根长度以及EE2浓度（P菌丝体Sp。GHH。这与土壤尿素酶和脱氢酶活性的变化一致，表明植物培养刺激了根茎的生长微生物。与L. Perenne结合使用的杂种菌EE2污染的土壤和植物 - 微生物相互作用对EE2去除具有协同作用。
PurposeThe increasing land application of sewage sludge and animal manure has accelerated the input of 17α-ethynylestradiol (EE2) into soils and environment, which would influence the health of vegetables and also the hormonal system of animals. In order to remove EE2 from soils, one possible option is bioremediation by using EE2-degrading microorganism or/and EE2-accumulating plants. The study attempted to isolate EE2-degrading microorganism from soils, and investigate the effect of this microorganism and/or organic pollutant removable plant Lolium perenne on the removal of EE2 from soil.Materials and methodsEE2-degrading bacteria Hyphomicrobium sp. GHH was obtained from farmland soil. Pot experiments were taken to investigate the inoculation of Hyphomicrobium sp. GHH, cultivation of L. perenne, and inoculation of Hyphomicrobium sp. GHH + cultivation of L. perenne on the EE2 removal from soil. The plant biomass, EE2 concentration in plant root and shoot, phytoremediation efficiency, and the soil urease activity and dehydrogenase activity were determined to evaluate the interaction of plant and microbe on EE2 removal from soil.Results and discussionThe inoculation of Hyphomicrobium sp. GHH significantly increased root length of L. perenne as well as EE2 concentrations in root and shoot (P < 0.05). On days 14, 28, and 42 after the treatment with l. perenne cultivation alone, the phytoextraction efficiency of EE2 was 16.5, 25.2, and 29.8%, respectively, while this phytoextraction efficiency was improved by 82.0, 44.0, and 41.0%, respectively, with the inoculation of Hyphomicrobium sp. GHH. On days 28 and 42, the combination of Hyphomicrobium sp. GHH with l. perenne removed 94.0 and 99.0% of EE2, respectively, from the soil. EE2 was mainly stored in the root of L. perenne, with all the translocation factor (TF) values < 1. The promoting effect of different treatments on the removal efficiency of EE2 decreased in the order: L. perenne + Hyphomicrobium sp. GHH > L. perenne alone > Hyphomicrobium sp. GHH. This was consistent with the changing trend of the activities of soil urease and dehydrogenase, indicating that plant cultivation stimulated the growth of rhizospheric microorganisms.Conclusions GHH in combination with L. perenne has great potential for remediating EE2-contaminated soil, and plant-microbe interaction has synergistic effect on the EE2 removal.