The human progenitor cell antigen (CD34) is localized on endothelial cells, dermal dendritic cells, and perifollicular cells in formalin-fixed normal skin, and on proliferating endothelial cells and stromal spindle-shaped cells in Kaposi's sarcoma.
The human progenitor cell antigen (CD34) is localized on endothelial cells, dermal dendritic cells, and perifollicular cells in formalin-fixed normal skin, and on proliferating endothelial cells and stromal spindle-shaped cells in Kaposi's sarcoma.
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DOI：
10.1001/archderm.1991.04510010091009
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发表时间：
1991-04
影响因子：
--
通讯作者：
B. Nickoloff
中科院分区：
文献类型：
--
作者：
B. Nickoloff
人类祖细胞抗原（CD34）选择性地表达在骨髓中的造血祖细胞上。在速冻皮肤的低温切片或正常皮肤的福尔马林固定石蜡包埋切片中，抗 CD34 单克隆抗体对血管内皮细胞和血管周围/间质树突细胞进行免疫染色，特别是在网状真皮中。毛囊中部（即凸起区域）（即假定的毛囊干细胞的位置）中的毛囊周围纺锤形细胞的独特群体呈 CD34 阳性，小汗腺内部和周围的纺锤形细胞也强调其基底膜区域。在斑块/斑块和肿瘤阶段获得性免疫缺陷综合征相关的卡波西肉瘤病变中，增殖的内皮细胞和纺锤形基质细胞上都存在 CD34 表达。 CD34阳性内皮细胞和纺锤形基质细胞可能在正常和患病皮肤中发挥重要的参与和支持功能。
The human progenitor cell antigen (CD34) is selectively expressed on hematopoietic progenitor cells in the bone marrow. In either cryostat sections of snap-frozen skin, or formalin-fixed paraffin-embedded sections of normal skin, anti-CD34 monoclonal antibody immunostained vascular endothelial cells and perivascular/interstitial dendritic cells, particularly in the reticular dermis. A distinctive population of perifollicular spindle-shaped cells in the midportion of the follicle (ie, bulge area), which is the site of the putative hair follicle stem cells, were CD34 positive, as were spindle-shaped cells in and around the eccrine glands accentuating their basement membrane zone. In patch/plaque--and tumor-stage acquired immunodeficiency syndrome-associated Kaposi's sarcoma lesions, CD34 expression was present on both the proliferating endothelial cells as well as the spindle-shaped stromal cells. CD34 positive endothelial cells and spindle-shaped stromal cells may play important participatory and supportive functions in both normal and diseased skin.