Effect of Heating the Interface on Chromatographic Performance of Solid Phase Microextraction Coupled to High-performance Liquid Chromatography
Effect of Heating the Interface on Chromatographic Performance of Solid Phase Microextraction Coupled to High-performance Liquid Chromatography
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DOI：
10.1039/a704729k
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
H. Daimon;J. Pawliszyn
中科院分区：
文献类型：
--
作者：
H. Daimon;J. Pawliszyn
研究了固相微萃取与高效液相色谱联用中加热进样器的影响。用SPME纤维提取的分析物被引入到使用专门设计的SPME-HPLC接口的HPLC分离柱中，其中分析物用HPLC溶剂移动的相解吸。SPME纤维在该界面中被加热以增强解吸，其中加热丝使用直流电源或电容放电快速加热系统盘绕在腔室周围。温度升高降低了纤维涂层-溶剂分布常数，并增加了扩散速率，从而提高了从纤维的固定相到移动的相的传质速率。较高的解吸速率减小了进样带宽，并减小了峰增宽，从而增强了色谱性能。解吸温度被发现有一个显着的效果上的解吸和分离的效率。提高解吸温度显着减少携带的纤维和提高色谱分辨率。
The effect of heating the injector was investigated for solid phase microextraction (SPME) coupled to high-performance liquid chromatography (HPLC). Analytes extracted with an SPME fiber were introduced to a HPLC separation column using the specially designed SPME–HPLC interface, where the analytes were desorbed with HPLC solvent mobile phase. The SPME fiber was heated in this interface to enhance desorption with a heating wire coiled around the chamber using a dc power supply or a capacitive discharge quick heating system. A temperature increase reduces the fiber coating–solvent distribution constant, and increases diffusion rates, thereby enhancing the mass-transfer rate from the stationary phase of the fiber to the mobile phase. A higher desorption rate reduces injection band width, and reduces peak broadening, thus enhancing the chromatographic performance. Desorption temperature was found to have a pronounced effect on the efficiency of desorption and separation. Increasing the desorption temperature significantly reduced carryover on the fiber and enhanced the chromatographic resolution.