RAD3 protein of Saccharomyces cerevisiae is a DNA helicase.
RAD3 protein of Saccharomyces cerevisiae is a DNA helicase.
复制标题
酿酒酵母的 RAD3 蛋白是一种 DNA 解旋酶。
DOI：
10.1073/pnas.84.24.8951
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发表时间：
1987
影响因子：
11.1
通讯作者：
Prakash,S
中科院分区：
文献类型：
--
作者：
Sung,P;Prakash,L;Matson,SW;Prakash,S
酿酒酵母的RAD3基因是细胞活力和受损DNA的切除修复所必需的，它编码一个89 kda的蛋白，具有单链DNA依赖的atp酶活性。我们现在表明，RAD3蛋白还具有解旋酶活性，可以将71-851个核苷酸的DNA片段退火成环状单链M13 DNA构建的DNA底物中的双链区域解旋。DNA解旋酶的活性依赖于ATP的水解，其最适pH值约为5.6，并被针对大肠杆菌中产生的截断的RAD3蛋白的抗体所抑制。RAD3解旋酶沿5‘----3’方向沿单链DNA易位。RAD3解旋酶的运动方向与DNA复制过程中它在复制叉之前解旋DNA双链的可能性是一致的。
The Saccharomyces cerevisiae RAD3 gene, which is required for cell viability and excision repair of damaged DNA, encodes an 89-kDa protein that has a single-stranded DNA-dependent ATPase activity. We now show that the RAD3 protein also possesses a helicase activity that unwinds duplex regions in DNA substrates constructed by annealing DNA fragments of 71-851 nucleotides to circular, single-stranded M13 DNA. The DNA helicase activity is dependent on the hydrolysis of ATP, has a pH optimum of approximately 5.6, and is inhibited by antibodies raised against a truncated RAD3 protein produced in Escherichia coli. The RAD3 helicase translocates along single-stranded DNA in the 5'----3' direction. The direction of RAD3 helicase movement is consistent with the possibility that it unwinds DNA duplexes in advance of the replication fork during DNA replication.