Treatment of poplar callus with ferulic and sinapic acids I: incorporation and enhancement of lignin biosynthesis
Treatment of poplar callus with ferulic and sinapic acids I: incorporation and enhancement of lignin biosynthesis
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DOI：
10.1007/s10086-002-0477-7
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发表时间：
2003-10
影响因子：
2.9
通讯作者：
Katsuyoshi Hamada;Y. Tsutsumi;Kazuchika Yamauchi;K. Fukushima;T. Nishida
中科院分区：
文献类型：
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作者：
Katsuyoshi Hamada;Y. Tsutsumi;Kazuchika Yamauchi;K. Fukushima;T. Nishida
阿魏酸（FA）、四叔魏酸（DFA）、辛酸（SA）和七叔魏酸（DSA）分别外源供给杨树（Populus albaL.）愈伤组织。添加FA或SA使愈伤组织的木质素含量增加到对照的两倍左右。细胞壁残渣碱水解物的气相色谱分析表明，只有微量的SA与细胞壁结合，FA的含量不到总木质素的2%。对DFA处理的愈伤组织进行硫代酸分解，结果表明DFA有效地转化为针叶树醇和新树醇，并转化为相应的木质素。DSA未掺入丁香基木质素或愈创木基木质素中，但DSA处理愈伤组织可显著提高丁香基木质素硫代酸解产物的产量。这些结果表明，SA本身可能不是新颖醇和丁香基木质素的前体，但它可能诱导或促进杨树愈伤组织中丁香基木质素的生物合成。
Ferulic acid (FA), tetradeuteroferulic acid (DFA), sinapic acid (SA), or heptadeuterosinapic acid (DSA) was exogenously supplied to poplar (Populus albaL.) callus. Administration of FA or SA increased the lignin content of the callus to about twice that of the control callus. Gas chromatographic analysis of the alkali hydrolysate of the cell wall residue revealed that only a trace amount of SA was bound to the cell wall, and the amount of FA was less than 2% of the total callus lignin. Thioacidolysis of the DFA-treated callus indicated that DFA is effectively converted to both coniferyl and sinapyl alcohols and then incorporated into the corresponding lignin. Incorporation of DSA into syringyl lignin or guaiacyl lignin was not observed, but yields of syringyl lignin thioacidolysis products were markedly increased by DSA treatment of the callus. These results suggest that SA may not be a precursor of sinapyl alcohol and syringyl lignin per se, but it may induce or enhance the biosynthesis of syringyl lignin in poplar callus.