Construction of Dopamine-Releasing Gold Surfaces Mimicking Presynaptic Membrane by On-Chip Electrochemistry
Construction of Dopamine-Releasing Gold Surfaces Mimicking Presynaptic Membrane by On-Chip Electrochemistry
复制标题
通过片上电化学构建模拟突触前膜的释放多巴胺的金表面
DOI：
10.1021/jacs.9b01003
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发表时间：
2019-06-05
影响因子：
15
通讯作者：
Jiang, Xingyu
中科院分区：
文献类型：
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作者：
Li, Jun;Sun, Chun-Lin;Jiang, Xingyu
我们报告了一种策略，构建多巴胺释放金表面模仿突触前膜的微流控芯片上模拟在体神经信号。我们构建了多巴胺自组装单分子膜（DA SAMs）的电化学脱保护的甲基基团保护的DA SAMs在金表面。在金表面施加非水解负电位可实现DA自组装膜的电化学可控释放。我们的方法在构建DA SAM避免了聚合和质子化的DA分子，这可能会导致失败的DA SAM形成。通过结合微流体技术，我们实现了DA从金表面的电化学空间和时间可控释放。此外，通过在图案化的DA自组装膜上培养神经元，DA自组装膜与神经元之间的界面可以充当突触前膜，并且DA的时空释放可以高精度地调节神经元的活动。我们的研究在神经生物学研究和药物筛选领域具有很大的前景。
We report a strategy to construct a dopamine releasing gold surface mimicking a presynaptic membrane on a microfluidic chip to simulate in vivo neural signaling. We constructed dopamine self-assembled monolayers (DA SAMs) by electrochemical deprotection of methyl group-protected DA SAMs on a gold surface. Electrochemically controllable release of DA SAMs can be realized by applying nonhydrolytic negative potential on the gold surface. Our method in constructing DA SAMs avoids the polymerization and protonation of DA molecules which may lead to the failure of the DA SAM formation. By combining microfluidics, we realized spatial and temporal controllable release of DA by electrochemistry from the gold surface. Furthermore, by culturing neurons on the patterned DA SAMs, the interface between the DA SAMs and the neurons could serve as a presynaptic membrane, and the spatiotemporal release of DA could modulate the neuron activity with high precision. Our study holds great promise in the fields of neurobiology research and drug screening.