LONGITUDINAL-STUDY OF INVIVO WOUND REPAIR AND INVITRO CELLULAR SENESCENCE OF DERMAL FIBROBLASTS
LONGITUDINAL-STUDY OF INVIVO WOUND REPAIR AND INVITRO CELLULAR SENESCENCE OF DERMAL FIBROBLASTS
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DOI：
10.1016/0531-5565(91)90058-t
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发表时间：
1991-01-01
影响因子：
3.9
通讯作者：
DEAMOND, SF
中科院分区：
文献类型：
--
作者：
BRUCE, SA;DEAMOND, SF
进行纵向研究以证实先前在横截面研究中观察到的体内年龄与体外增殖能力之间的反比关系，并研究体外真皮成纤维细胞的生长与生理功能（即，伤口修复），已知其在体内随年龄而下降。 从1月龄开始，从12只雄性仓鼠的皮肤穿刺活检产生成纤维细胞培养物，此后间隔6个月，直到动物自然死亡。 来自所有个体的所有培养物均表现出有限的增殖能力，并且观察到供体年龄与最大体外增殖能力之间呈反比关系。 此外，观察到真皮成纤维细胞的体外增殖能力与活检部位的修复效率之间存在直接相关性。 然而，体外增殖能力和体内伤口修复效率的这些变化在12-18个月龄之后没有进展，并且在个体的最终寿命的任何年龄都没有指示。 这项研究提供的证据表明，在体外增殖能力的真皮成纤维细胞和在体内伤口修复可能是具有共同机制的可比现象。 然而，这些现象与个体的最终寿命之间的非渐进性和缺乏相关性表明，它们作为衰老的生物标志物的用途仅限于比物种平均寿命更年轻的动物。
A longitudinal study was performed to confirm the inverse relationship between in vivo age and in vitro proliferative capacity previously observed in a cross-sectional study, and to investigate the relationship between the growth of dermal fibroblasts in vitro and a physiological function (i.e., wound repair) that is known to decline with age in vivo. Fibroblast cultures were generated from skin punch biopsies from 12 male hamsters beginning at 1 month of age and at 6-months interval thereafter until the natural death of the animal. All cultures from all individuals exhibited finite proliferative capacity, and an inverse relationship was observed between donor age and maximum in vitro proliferative capacity. In addition, a direct correlation between the in vitro proliferative capacity of the dermal fibroblasts in vitro and the repair efficiency of the biopsy site was observed. However, these changes in the in vitro proliferative capacity and in vivo wound repair efficiency were not progressive beyond 12-18 months of age and were not indicative at any age of an individual's ultimate lifespan. This study provides evidence that in vitro proliferative capacity of dermal fibroblasts and in vivo wound repair may be comparable phenomena that share a common mechanism. However, the nonprogressive nature and the lack of correlation between these phenomena and the individual's ultimate lifespan indicate that their use as biological markers of aging is limited to animals younger than the mean lifespan of the species.