Effects of nanobubble collapse on cell membrane integrity.
Effects of nanobubble collapse on cell membrane integrity.
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DOI：
10.1142/s2424913017500084
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发表时间：
2017-06-01
影响因子：
--
通讯作者：
Wang, Xianqiao
中科院分区：
文献类型：
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作者：
Becton, Matthew;Averett, Rodney;Wang, Xianqiao
最近的研究表明，超声可用于打开载药脂质体以释放其有效载荷；然而，足以破坏脂质膜的冲击波会对周围细胞造成附带损害。类似地，可用于破裂细胞膜以溶解细胞的破坏性冲击波（例如，在癌症治疗中）也可能损害或破坏附近的健康组织。为了解决这个问题，我们使用耗散粒子动力学（DPD）模拟来研究在冲击波和模型细胞膜之间添加空化泡以改变冲击波前缘，从而允许低速冲击波特异性地破坏预定目标。我们特别关注一个球形脂质双层模型，并注意到冲击波速度、气泡大小和方向对模型细胞损伤的影响。我们表明，空化泡大大降低了破坏脂质双分子层和破坏模型细胞所需的冲击波速度。空化泡将冲击波锋面的动能集中到一个较小的区域，在模型胞体的边缘诱导穿透。通过这项工作，我们提供了一种全面的方法来研究通过冲击波冲击模型细胞破坏的复杂性，并希望为利用诱导空化泡和低速冲击波启动靶向细胞破坏提供指导。
Recent studies have shown that ultrasound is used to open drug-carrying liposomes to release their payloads; however, a shockwave energetic enough to rupture lipid membranes can cause collateral damage to surrounding cells. Similarly, a destructive shockwave, which may be used to rupture a cell membrane in order to lyse the cell (e.g., as in cancer treatments) may also impair or destroy nearby healthy tissue. To address this problem, we use dissipative particle dynamic (DPD) simulation to investigate the addition of a cavitation bubble between the shockwave and the model cell membrane to alter the shockwave front, allowing low-velocity shockwaves to specifically damage an intended target. We focus specifically on a spherical lipid bilayer model, and note the effect of shockwave velocity, bubble size, and orientation on the damage to the model cell. We show that a cavitation bubble greatly decreases the necessary shockwave velocity required to damage the lipid bilayer and rupture the model cell. The cavitation bubble focuses the kinetic energy of the shockwave front into a smaller area, inducing penetration at the edge of the model cell. With this work, we provide a comprehensive approach to the intricacies of model cell destruction via shockwave impact, and hope to offer a guideline for initiating targeted cellular destruction using induced cavitation bubbles and low-velocity shockwaves.