Rapid and efficient immobilization of soluble and small particulate antigens for solid phase radioimmunoassays.
Rapid and efficient immobilization of soluble and small particulate antigens for solid phase radioimmunoassays.
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快速有效地固定可溶性和小颗粒抗原，用于固相放射免疫测定。
DOI：
10.1080/15321818108056972
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
D. Worthen
中科院分区：
文献类型：
--
作者：
P. Cleveland;M. Wickham;M. Goldbaum;A. F. Ryan;D. Worthen
采用快速抗原固定法（10 min.）用可溶性抗原（牛血清白蛋白、表皮生长因子和山羊IgG）和小颗粒抗原（匙孔血蓝蛋白和E.大肠杆菌）。该技术导致高百分比的可溶性抗原保持牢固结合，山羊IgG（89%）、牛血清白蛋白（73%）。用125I标记的葡萄球菌蛋白A放射免疫分析法检测，我们测试的所有抗原在干燥后保留了它们的抗原性。将这种抗原固定方法与吸附到塑料威尔斯孔中进行比较，发现其更快（10分钟对18小时），并且比吸附到塑料威尔斯孔中的效率高5倍。使用这种技术，我们能够检测到低至40 ng的牛血清白蛋白。这些特征表明，这种可溶性抗原固定化技术可用于快速检测针对许多不同抗原的抗体，以及直接检测ng量的抗原。
A rapid method of antigen immobilization (10 min.) was developed using soluble antigens (bovine serum albumin, epidermal growth factor, and goat IgG) and small particulate antigens (Keyhole limpet hemocynanine and E. coli) by drying them on filter paper discs. This technique results in a high % of the soluble antigen remaining firmly bound, goat IgG (89%), bovine serum albumin (73%). All the antigens we tested retained their antigenicity after drying as detected by [125I] labeled staphylococcal protein A radioimmunoassay. This method of antigen immobilization was compared to adsorption to plastic wells and was found to be much faster (10 min vs 18 hrs) and was 5 times more efficient than adsorption to plastic wells. Using this technique, we were able to detect as little as 40 ng of bovine serum albumin. These characteristics suggest that this technique of soluble antigen immobilization may be useful in rapid detection of antibodies to many different antigens, as well as detecting ng amounts of the antigens directly.