Bacillus subtilis attachment to Aspergillus niger hyphae results in mutually altered metabolism
Bacillus subtilis attachment to Aspergillus niger hyphae results in mutually altered metabolism
复制标题
DOI：
10.1111/1462-2920.12564
复制
发表时间：
2015-06-01
影响因子：
5.1
通讯作者：
Kovacs, Akos T.
中科院分区：
文献类型：
--
作者：
Benoit, Isabelle;van den Esker, Marielle H.;Kovacs, Akos T.
微生物之间的相互作用影响微生物群落成员的生长、代谢和分化。虽然直接和间接的竞争，如拮抗作用和营养消耗对种群中相互作用的成员有负面影响，但微生物在自然界中也进化得不仅能战斗，而且在某些情况下能相互适应或支持，同时增加群落的适应性。土壤中细菌和真菌的存在导致各种相互作用，包括互利共生。芽孢杆菌附着在植物根部，在根际形成复杂的群落。枯草芽孢杆菌在尼日尔曲霉存在下生长时，通过附着并生长在菌丝上与真菌类似地相互作用。基于在双转录组实验中获得的数据，我们认为真菌和细菌在这种相互作用过程中改变了它们的代谢。有趣的是，当细菌附着在菌丝体上时，分别与B.subtilis和A.尼日尔的抗真菌和推定的抗细菌防御机制相关的基因的转录降低。对培养物上清液的分析表明，当细菌与真菌共培养时，枯草B.subtilis的表面活性素产生减少。我们的实验为细菌和真菌之间的相互作用提供了新的见解。
Interaction between microbes affects the growth, metabolism and differentiation of members of the microbial community. While direct and indirect competition, like antagonism and nutrient consumption have a negative effect on the interacting members of the population, microbes have also evolved in nature not only to fight, but in some cases to adapt to or support each other, while increasing the fitness of the community. The presence of bacteria and fungi in soil results in various interactions including mutualism. Bacilli attach to the plant root and form complex communities in the rhizosphere. Bacillus subtilis, when grown in the presence of Aspergillus niger, interacts similarly with the fungus, by attaching and growing on the hyphae. Based on data obtained in a dual transcriptome experiment, we suggest that both fungi and bacteria alter their metabolism during this interaction. Interestingly, the transcription of genes related to the antifungal and putative antibacterial defence mechanism of B.subtilis and A.niger, respectively, are decreased upon attachment of bacteria to the mycelia. Analysis of the culture supernatant suggests that surfactin production by B.subtilis was reduced when the bacterium was co-cultivated with the fungus. Our experiments provide new insights into the interaction between a bacterium and a fungus.