Androgen receptor binding to nuclear matrix in vitro and its inhibition by 8S androgen receptor promoting factor.
Androgen receptor binding to nuclear matrix in vitro and its inhibition by 8S androgen receptor promoting factor.
复制标题
雄激素受体体外与核基质的结合及其受8S雄激素受体促进因子的抑制。
DOI：
10.1021/bi00310a015
复制
发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Wilson,EM
中科院分区：
文献类型：
--
作者：
Colvard,DS;Wilson,EM
Douglas S. Colvard 和 Elizabeth M. Wilson** 摘要：部分纯化的 4.5 S [3H] 二氢睾酮受体与从大鼠 Dunning 前列腺肿瘤中分离出的核基质结合，其特性与报道的完整细胞核中雄激素受体结合的特性相似 [Colvard, D. S., & Wilson, E. M.(1984) Biochemistry（本期前一篇论文）] 需要 Zn2+ 和巯基乙醇，是可饱和的，并且具有温度依赖性和高亲和力 (Xa~ 1013 M-1)。在毫克 DNA 当量基础上，雄激素受体的基质结合程度（700 fmol 受体结合/毫克基质蛋白）与完整细胞核相似，相当于约 1400 个位点/细胞核。 4.5 S 雄激素受体在 0、15 和 25 C 下与基质结合的结合速率常数 (fca) 分别为 2.7 X 105、1.2 X 106 和 2.4 X 106 M“'min-1，表明能量为
Douglas S. Colvard and Elizabeth M. Wilson** abstract: The partially purified 4.5 S [3H] dihydrotestosterone receptor binds to nuclear matrix isolated from rat Dunning prostate tumor with properties similar to those reported for androgen receptor bindingin intact nuclei [Colvard, D. S., & Wilson, E. M.(1984) Biochemistry (preceding paper in this issue)] in that it requires Zn2+ and mercaptoethanol, is sat-urable, and is temperature dependent and of high affinity (Xa~ 1013 M-1). On a milligrams of DNA equivalent basis, the extent of matrix binding of androgen receptor (700 fmol of receptor bound/mgof matrix protein) is similar to that of intact nuclei, corresponding to~ 1400 sites/nucleus. Asso-ciation rate constants (fca) for 4.5 S androgen receptor binding to matrix at 0, 15, and 25 C are 2.7 X 105, 1.2 X 106, and 2.4 X 106 M “'min-1, respectively, indicating an energy of