Carbon and clay nanoparticles induce minimal stress responses in gram negative bacteria and eukaryotic fish cells
Carbon and clay nanoparticles induce minimal stress responses in gram negative bacteria and eukaryotic fish cells
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DOI：
10.1002/tox.21824
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发表时间：
2014-08
影响因子：
4.5
通讯作者：
Alicia A. Taylor;G. M. Aron;G. Beall;N. Dharmasiri;Yixin Zhang;R. McLean
中科院分区：
文献类型：
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作者：
Alicia A. Taylor;G. M. Aron;G. Beall;N. Dharmasiri;Yixin Zhang;R. McLean
我们在体外研究了两种粘土基纳米颗粒Clopartite ® Na+（Clopartite）和埃洛石以及聚合物复合材料行业常用的多壁碳纳米管（MWCNT）的潜在致突变和毒性作用。使用艾姆斯试验，三种纳米颗粒没有真正的致突变作用，尽管肠道沙门氏菌变种的生长。鼠伤寒沙门氏菌（S.typhimurium）在较高的纳米颗粒浓度下减少。我们研究了纳米颗粒对大肠杆菌和S.鼠伤寒沙门氏菌，包括oxyR和rpoS突变体，它们对氧化应激敏感。在纳米颗粒的存在下，当有氧生长与光的oxyR突变体被抑制。在无光或厌氧生长期间未观察到毒性。E.大肠杆菌rpoS突变体显示出一定的毒性时，培养与Cloprotein和多壁碳纳米管只有当生长有氧与光。其他纳米颗粒或S.鼠伤寒rpoS突变体。MWCNT仅在测试的最高浓度下对丘疹性上皮瘤（EPC）细胞表现出轻微的毒性作用。粘土纳米颗粒对EPC细胞没有明显的毒性。我们得出结论，粘土基纳米颗粒和多壁碳纳米管不会产生致突变作用，并且在所有细菌物种或原核细胞和真核细胞之间不具有一般毒性作用。在测试的最高浓度下，仅在真核EPC细胞中观察到对MWCNT的适度毒性。在细菌菌株中观察到对粘土基和MWCNT纳米颗粒的有限种属特异性毒性，主要是由于加剧氧化应激的培养条件和突变。© 2012 Wiley Periodicals，Inc.环境毒理学29：961-968，2014年。
We investigated in vitro the potential mutagenic and toxic effects of two clay‐based nanoparticles, Cloisite® Na+ (Cloisite) and halloysite; and multi‐walled carbon nanotubes (MWCNT), commonly used in the polymer composite industry. Using the Ames test, the three nanoparticles did not have a true mutagenic effect, although growth of Salmonella enterica var. Typhimurium (S.typhimurium) was diminished at higher nanoparticle concentrations. We investigated the impact of nanoparticles on Escherichia coli and S. typhimurium including oxyR and rpoS mutants, which are susceptible to oxidative stress. The oxyR mutants were inhibited in the presence of nanoparticles, when grown aerobically with light. Toxicity was not observed in the absence of light or during anaerobic growth. E. coli rpoS mutants exhibited some toxicity when cultured with Cloisite and MWCNT only when grown aerobically with light. There was no effect with other nanoparticles, or with S. typhimurium rpoS mutants. MWCNT exhibited a slight toxic effect against Epithelioma papulosum cyprini (EPC) cells only at the highest concentration tested. There was no discernable toxicity to EPC cells caused by the clay nanoparticles. We conclude that clay‐based nanoparticles and MWCNT do not exert a mutagenic effect and do not have a general toxic effect across all bacterial species or between prokaryotic and eukaryotic cells. Modest toxicity was only observed in eukaryotic EPC cells against MWCNT at the highest concentration tested. Limited species‐specific toxicity to clay based and MWCNT nanoparticles was seen in bacterial strains primarily due to culture conditions and mutations that exacerbate oxidative stress. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 961–968, 2014.