Neutrophil transepithelial migration: Evidence for sequential, contact-dependent signaling events and enhanced paracellular permeability independent of transjunctional migration
Neutrophil transepithelial migration: Evidence for sequential, contact-dependent signaling events and enhanced paracellular permeability independent of transjunctional migration
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DOI：
10.4049/jimmunol.169.1.476
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发表时间：
2002-07-01
影响因子：
4.4
通讯作者：
Parkos, CA
中科院分区：
文献类型：
--
作者：
Edens, HA;Levi, BP;Parkos, CA
多形核白细胞(PMN)通过肠隐窝上皮细胞的活跃迁移是炎症性肠病的一个特征，并与患者的症状相关。以往的体外研究表明，PMN跨上皮迁移导致上皮通透性增加。在这项研究中，我们模拟了PMN跨T84单分子层的迁移，并证明了在没有跨上皮迁移的情况下，细胞旁对小溶质的通透性增加，但需要PMN与上皮细胞基侧膜接触和跨上皮趋化梯度。在PMN进入细胞旁间隙之前发生的早期事件包括对小溶质的通透性增加(
Active migration of polymorphonuclear leukocytes (PMN) through the intestinal crypt epithelium is a hallmark of inflammatory bowel disease and correlates with patient symptoms. Previous in vitro studies have shown that PMN transepithelial migration results in increased epithelial permeability. In this study, we modeled PMN transepithelial migration across T84 monolayers and demonstrated that enhanced paracellular permeability to small solutes occurred in the absence of transepithelial migration but required both PMN contact with the epithelial cell basolateral membrane and a transepithelial chemotactic gradient. Early events that occurred before PMN entering the paracellular space included increased permeability to small solutes (