Parameter-specific analgesic effects of electroacupuncture mediated by degree of regulation TRPV1 and P2X3 in inflammatory pain in rats
Parameter-specific analgesic effects of electroacupuncture mediated by degree of regulation TRPV1 and P2X3 in inflammatory pain in rats
复制标题
TRPV1和P2X3调节程度介导的电针对大鼠炎性痛的参数特异性镇痛作用
DOI：
10.1016/j.lfs.2018.03.028
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发表时间：
2018-05-01
期刊：
影响因子：
6.1
通讯作者：
Liu, B. Y.
中科院分区：
文献类型：
--
作者：
Fang, J. Q.;Du, J. Y.;Liu, B. Y.
目的：观察不同刺激次数和频率EA对CFA注射后DRG中TRPV1和P2X3表达水平介导的痛觉过敏的参数特异性抗痛觉过敏作用。主要方法：大鼠右后爪注射CFA造模。双侧ST36和BL60行EA治疗。分别用Von Frey灯丝和辐射热源测试足部戒断阈值（PWT）和足部戒断潜伏期（PWL）。免疫荧光和western blot检测TRPV1和P2X3的表达。进一步利用α - β -肉atp和辣椒素联合EA研究PWL的变化。主要发现：不同刺激时间（20、30、45 min）联合不同频率（2 Hz、100 Hz、2/100 Hz）的电刺激对PWT和PWL均有镇痛作用；然而，EA的镇痛效果水平主要与EA频率相关。100 Hz时EA的镇痛效果优于2 Hz。100hz EA对DRG中TRPV1和P2X3的调控水平高于2hz。此外，TRPV1激动剂和P2X3激动剂均可损害EA镇痛水平。
Aims: Observing the parameter-specific anti-hyperalgesic effects of EA with different stimulation times and frequencies on painful hyperalgesia mediated by the level of TRPV1 and P2X3 expression in DRG after CFA injection.Main methods: The model was induced by the injection of CFA in each rat's right hind paw. EA treatment was applied to the bilateral ST36 and BL60. Paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) were tested with Von Frey filaments and the radiant heat source of the test instrument, respectively. TRPV1 and P2X3 expressions were measured by immunofluorescence and western blot. alpha beta-meATP and capsaicine combined with EA were further utilized to investigate the change in PWL.Key findings: Different stimulation times (20, 30, 45 min) combined with different frequencies (2 Hz, 100 Hz, 2/100 Hz) of EA have analgesic effects on the PWT and PWL; however, the level of the hypoalgesic efficacy of EA was primarily associated with EA frequency. The analgesic effect of EA was better at 100 Hz than at 2 Hz. The level of regulation of 100 Hz EA on TRPV1 and P2X3 in DRG was greater than that of 2 Hz. Furthermore, both TRPV1 agonist and P2X3 agonist may impair the level of EA analgesia.