Inducible processing of interferon regulatory factor-2.
Inducible processing of interferon regulatory factor-2.
复制标题
干扰素调节因子2的诱导加工。
DOI：
10.1128/mcb.12.8.3325-3336.1992
复制
发表时间：
1992
影响因子：
5.3
通讯作者：
Maniatis,T
中科院分区：
文献类型：
--
作者：
Palombella,VJ;Maniatis,T
PRDI-BFc和PRDI-BFi是特异性结合病毒诱导人β干扰素（IFN-β）基因所需的调节元件的蛋白质。PRDI-BFc具有组成型结合活性，而PRDI-BFi的结合活性只有在细胞被病毒或聚(I)·聚(C)加环己亚胺等诱导剂处理后或在某些细胞中被环己亚胺单独处理后才能观察到。在本文中，我们报道了PRDI-BFc是干扰素调节因子-2 (IRF-2)，一种已知的转录抑制因子。此外，我们发现PRDI-BFi是IRF-2的截断形式，缺少大约185个c端氨基酸。因此，PRDI-BFi似乎是通过诱导蛋白水解产生的。虽然PRDI-BFc/IRF-2对IFN-β启动子的亲和力似乎不受c端氨基酸去除的影响，但在共转染实验中，PRDI-BFi作为抑制因子的能力明显低于完整的IRF-2。研究表明，IRF-2可以阻断转录激活因子IRF-1的活性，IRF-1也特异性结合IFN-β基因启动子。因此，IRF-2的诱导蛋白水解可能参与IFN-β基因或其他基因的调控，其中IRF-1与IRF-2的比例可以影响转录水平。
PRDI-BFc and PRDI-BFi are proteins that bind specifically to a regulatory element required for virus induction of the human beta interferon (IFN-β) gene. PRDI-BFc is a constitutive binding activity, while the PRDI-BFi binding activity is observed only after cells are treated with inducers such as virus or poly(I) · poly(C) plus cycloheximide or in some cells by cycloheximide alone. In this paper we report that PRDI-BFc is interferon regulatory factor-2 (IRF-2), a known transcriptional repressor. In addition, we find that PRDI-BFi is a truncated form of IRF-2, lacking approximately 185 C-terminal amino acids. Thus, PRDI-BFi appears to be generated by inducible proteolysis. Although the affinity of PRDI-BFc/IRF-2 for the IFN-β promoter does not appear to be affected by the removal of C-terminal amino acids, the ability of PRDI-BFi to function as a repressor in cotransfection experiments is significantly less than that of intact IRF-2. Studies have shown that IRF-2 can block the activity of the transcriptional activator IRF-1, which also binds specifically to the IFN-β gene promoter. Thus, the inducible proteolysis of IRF-2 may be involved in the regulation of the IFN-β gene or of other genes in which the ratio of IRF-1 to IRF-2 can affect the level of transcription.