Citrate synthase gene comparison, a new tool for phylogenetic analysis, and its application for the Rickettsiae
Citrate synthase gene comparison, a new tool for phylogenetic analysis, and its application for the Rickettsiae
复制标题
DOI：
10.1099/00207713-47-2-252
复制
发表时间：
1997-04-01
期刊：
影响因子：
--
通讯作者：
Raoult, D
中科院分区：
文献类型：
--
作者：
Roux, V;Rydkina, E;Raoult, D
应用PCR和自动激光荧光DNA测序仪，对28株立克次体的柠檬酸脱氢酶基因（gltA）进行了扩增和测序。序列比较分析表明，斑点热群立克次体大部分属于两个亚群之一，第一亚群包括立克次体Bar 29株，立克次体Rhipicephali，第二亚群较大，包括大多数人类病原体和仅从蜱中分离的立克次体;该亚群的成员是S株、非洲立克次体、蒙古立克次体、西伯利亚立克次体、帕克立克次体、康氏立克次体、立克次体，泰国斑疹伤寒立克次体，以色列斑疹伤寒立克次体、阿斯特拉罕热立克次体、斯洛伐克立克次体和日本立克次体。加拿大立克次体、贝氏立克次体和AB菌可能代表三个新的群。我们比较了柠檬酸合成酶基因序列和16 S rDNA序列的系统发育树。对于立克次体的遗传学，柠檬酸合酶的方法是更合适的，证明了显着的自举值的所有节点，除了那些在上面定义的较大的亚组。我们还比较了系统发育分析结果，这些结果是在对确定gltA序列的所有代表性结构域细菌的两种基因的序列进行比较中获得的。我们认为，gltA序列的比较可能是一种互补的方法，16 S rDNA测序推断细菌的进化，特别是当不稳定的系统发育模型，从核糖体序列，因为高水平的序列相似性之间的细菌研究。
Using PCR and an automated laser fluorescent DNA sequencer, we amplified and sequenced a 1,234-bp fragment of the citrate synthase-encoding gene (gltA) of 28 bacteria belonging to the genus Rickettsia, Comparative sequence analysis showed that most of the spotted fever group (SFG) rickettsiae belonged to one of two subgroups, The first subgroup included Rickettsia massiliae, strain Bar 29, Rickettsia rhipicephali, ''Rickettsia aeschlimanni,'' and Rickettsia montana, which have been isolated only from ticks, The second subgroup was larger and included the majority of the human pathogens and also rickettsiae isolated only from ticks; the members of this subgroup were strain S, Rickettsia africae, ''Rickettsia mongolotimonae,'' Rickettsia sibirica, Rickettsia parkeri, Rickettsia conorii, Rickettsia rickettsii, the Thai tick typhus rickettsia, the Israeli tick typhus rickettsia, the Astrakhan fever rickettsia, ''Rickettsia slovaca,'' and Rickettsia japonica, The sequence analysis also showed that the tick-borne organisms Rickettsia helvetica and Rickettsia australis and the mite-borne organism Rickettsia akari were associated with the SFG cluster; that Rickettsia prowazekii and Rickettsia typhi, two representatives of the typhus group, clustered together; and that Rickettsia canada, Rickettsia bellii, and the AB bacterium probably represent three new groups, We compared the phylogenetic trees inferred from citrate synthase gene sequences and from 16S ribosomal DNA (rDNA) sequences. For rickettsial phylogeny, the citrate synthase approach was more suitable, as demonstrated by significant bootstrap values for all of the nodes except those in the larger subgroup defined above. We also compared phylogenetic analysis results obtained in a comparison of the sequences of both genes for all of the representatives of the domain Bacteria for which the gltA sequence was determined. We believe that comparison of gltA sequences could be a complementary approach to 16S rDNA sequencing for inferring bacterial evolution, especially when unstable phylogenetic models are obtained from ribosomal sequences because of high levels of sequence similarity between the bacteria studied.