Determination of parasitic load in different tissues of murine toxoplasmosis after immunization by excretory-secretory antigens using Real time QPCR
Determination of parasitic load in different tissues of murine toxoplasmosis after immunization by excretory-secretory antigens using Real time QPCR
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DOI：
10.1016/j.exppara.2014.05.008
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发表时间：
2014-08-01
影响因子：
2.1
通讯作者：
Rasouli, Mehdi
中科院分区：
文献类型：
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作者：
Daryani, Ahmad;Sharif, Mehdi;Rasouli, Mehdi
弓形虫的排泄性分泌抗原（ESA）是针对弓形虫病的免疫候选者之一。为了评估免疫接种，我们确定了 ESA 免疫小鼠不同组织中弓形虫分布和寄生虫负荷的动力学。在这项实验研究中，病例组 (n = 18) 和对照组 (n = 18) 的 36 只小鼠分别用 ESA 和 PBS 进行免疫。 2周后，用弓形虫毒力RH菌株腹膜内攻击小鼠。攻击后（死亡前1、2、3天和最后一天）每天收集血液和不同组织（脑、脾、肝、心、肾和肌肉）。使用针对 B1 基因的实时 QPCR 计算寄生虫负载。 ESA作为疫苗在不同组织中表现出不同的效果。然而，与对照组相比，接受疫苗的感染小鼠血液和组织中的寄生虫负荷显着下降（P = 0.000）。这些结果表明，能够减少宿主不同组织中寄生虫负载的 ESA 可以作为开发针对弓形虫病的免疫策略的可评估候选者。 (C) 2014 Elsevier Inc. 保留所有权利。
Excretory secretory antigens (ESAs) of Toxoplasma gondii are one of the candidates for immunization against toxoplasmosis. For evaluation of immunization, we determined the kinetics of the distribution of Toxoplasma and parasite load in different tissues of mice immunized by ESAs. In this experimental study, 36 mice in case (n = 18) and control (n = 18) groups were immunized with ESAs and PBS, respectively. After 2 weeks, mice were challenged intraperitoneally with Toxoplasma virulent RH strain. Blood and different tissues (brain, spleen, liver, heart, kidney, and muscle) were collected daily after challenge (1, 2,3 and last day before death). Parasite load was calculated using Real time QPCR targeted at the B1 gene. ESAs as vaccine in different tissues showed various effects. However, infected mice which received the vaccine in comparison with control group, displayed a drastically decreasing in parasite burden, in their blood and tissues (P = 0.000). These results indicated that ESAs with reduction of parasite load in different tissues of host could be evaluable candidate for the development of immunization strategies against toxoplasmosis. (C) 2014 Elsevier Inc. All rights reserved.