Refinement of the Primate Corticospinal Pathway During Prenatal Development
Refinement of the Primate Corticospinal Pathway During Prenatal Development
复制标题
灵长类动物产前发育过程中皮质脊髓通路的完善
DOI：
10.1093/cercor/bhz116
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发表时间：
2019
期刊：
影响因子：
3.7
通讯作者：
Kennedy, Henry
中科院分区：
文献类型：
--
作者：
Ribeiro Gomes, Ana Rita;Olivier, Etienne;Killackey, Herbert P;Giroud, Pascale;Berland, Michel;Knoblauch, Kenneth;Dehay, Colette;Kennedy, Henry
皮质脊髓（CS）通路发育细化的扰动导致运动障碍。虽然非灵长类动物的发育改进有很好的记录，但在灵长类动物中，对发育中的CS通路的侵入性研究仅限于新生儿和出生后阶段，此时改进相对温和。本研究研究了食蟹猴CS投射神经元在发育过程中的分布变化。在胚胎日脊髓颈椎水平注射逆行示踪剂(E) 95和E105显示：(i)反向标记神经元的面积分布比新生儿更广泛，在前额叶、边缘、颞叶和枕叶皮层发现密集的标记；（ii）对侧和同侧凸出的CS神经元的分布在位置和标记神经元的数量上是相似的，而成人的对侧投影比同侧投影高一个数量级。从一个很大程度上限制注射的结果表明，迄今为止未被怀疑的灰质早期神经支配。在胎儿中，杏仁核中央核、下丘脑、丘脑下核和相邻的无尾带区域也有密集的标记，在成人对照中，皮质下结构只有少量的投射。
Perturbation of the developmental refinement of the corticospinal (CS) pathway leads to motor disorders. While non-primate developmental refinement is well documented, in primates invasive investigations of the developing CS pathway have been confined to neonatal and postnatal stages when refinement is relatively modest. Here, we investigated the developmental changes in the distribution of CS projection neurons in cynomolgus monkey (Macaca fascicularis). Injections of retrograde tracer at cervical levels of the spinal cord at embryonic day (E) 95 and E105 show that: (i) areal distribution of back-labeled neurons is more extensive than in the neonate and dense labeling is found in prefrontal, limbic, temporal, and occipital cortex; (ii) distributions of contralateral and ipsilateral projecting CS neurons are comparable in terms of location and numbers of labeled neurons, in contrast to the adult where the contralateral projection is an order of magnitude higher than the ipsilateral projection. Findings from one largely restricted injection suggest a hitherto unsuspected early innervation of the gray matter. In the fetus there was in addition dense labeling in the central nucleus of the amygdala, the hypothalamus, the subthalamic nucleus, and the adjacent region of the zona incerta, subcortical structures with only minor projections in the adult control.