Small and Stable Sulfobetaine Zwitterionic Quantum Dots for Functional Live-Cell Imaging
Small and Stable Sulfobetaine Zwitterionic Quantum Dots for Functional Live-Cell Imaging
复制标题
DOI：
10.1021/ja1005493
复制
发表时间：
2010-04-07
影响因子：
15
通讯作者：
Dubertret, Benoit
中科院分区：
文献类型：
--
作者：
Muro, Eleonora;Pons, Thomas;Dubertret, Benoit
我们已经开发了一种新的表面涂层的半导体量子点（QD）的基础上的异双功能配体，克服了以前的限制，这些荧光探针在生物成像应用。在这里，我们表明，量子点封端的双齿两性离子二氢硫辛酸-磺基甜菜碱（DHLA-SB）配体是一个有利的替代聚乙二醇包覆的纳米粒子，因为它们结合了联合收割机小尺寸，低非特异性吸附，保存的光学性能，并随着时间的推移和pH值和盐度的宽范围内的优异的稳定性。此外，这些QD可以容易地用生物分子如链霉亲和素（SA）和生物素官能化。我们应用链霉亲和素功能化的DHLA-SB量子点来跟踪活细胞中大麻素受体1（CB 1 R）的细胞内再循环。这些量子点通过SA-生物素相互作用选择性地识别细胞表面的受体库，非特异性吸附可忽略不计。量子点保留了它们的光学性质，允许CB 1 R内化到内体中。此外，细胞活性明显不受探针的影响。
We have developed a novel surface coating for semiconductor quantum dots (QDs) based on a heterobifunctional ligand that overcomes most of the previous limits of these fluorescent probes in bioimaging applications. Here we show that QDs capped with bidentate zwitterionic dihydrolipoic acid-sulfobetaine (DHLA-SB) ligands are a favorable alternative to polyethylene glycol-coated nanoparticles since they combine small sizes, low nonspecific adsorption, preserved optical properties, and excellent stability over time and a wide range of pH and salinity. Additionally, these QDs can easily be functionalized with biomolecules such as streptavidin (SA) and biotin. We applied streptavidin-functionalized DHLA-SB QDs to track the intracellular recycling of cannabinoid receptor 1 (CB1R) in live cells. These QDs selectively recognized the pool of receptors at the cell surface via SA-biotin interactions with negligible nonspecific adsorption. The QDs retained their optical properties, allowing the internalization of CB1R into endosomes to be followed. Moreover, the cellular activity was apparently unaffected by the probe.