ISLET AUTO-TRANSPLANTATION IN THE PANCREATECTOMIZED DOG - EFFECT OF TIME ON GRAFT FUNCTION
ISLET AUTO-TRANSPLANTATION IN THE PANCREATECTOMIZED DOG - EFFECT OF TIME ON GRAFT FUNCTION
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DOI：
10.1007/bf01654944
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发表时间：
1984-01-01
影响因子：
2.6
通讯作者：
JOHNSTON, IDA
中科院分区：
文献类型：
--
作者：
ALDERSON, D;FARNDON, JR;JOHNSTON, IDA
在大型实验性糖尿病动物中的胰腺移植维持空腹血糖，但不能达到正常的葡萄糖耐量。这种和其他激素和代谢缺陷影响这些移植物控制或逆转糖尿病并发症的功效。本研究的目的是观察这种缺陷是否存在，以及它们如何随着时间的推移而改变。12只狗在接受胶原酶分散的胰腺碎片脾内自体移植后1个月和3个月进行了研究。33只正常动物作为对照。在空腹状态和静脉葡萄糖负荷后测定血糖、乳酸、丙酮酸、丙氨酸、甘油、3-羟基丁酸、胆固醇、游离脂肪酸、胰岛素、胰高血糖素和皮质醇。1个月时，葡萄糖清除率（K）降低，甘油、胆固醇、游离脂肪酸和3-羟基丁酸盐的空腹水平升高。在3个月时，K发生了显着改善，中间代谢恢复正常。在1至3个月之间，外周血中测得的葡萄糖估计胰岛素输出量显著增加。胰高血糖素和皮质醇在整个研究过程中均正常。与节段性胰腺移植不同，胰岛移植物随时间推移显示出功能改善，这一特征对未来的临床研究具有相当重要的意义。
Pancreatic transplants in large experimental diabetic animals maintain fasting euglycemia, but do not achieve normal glucose tolerance. This and other hormonal and metabolic defects influence the efficacy of these grafts to control or reverse the complications of diabetes. The aim of this study was to see if such defects exist, and how they might alter with time.Twelve dogs were studied 1 and 3 months after receiving intrasplenic autotransplants of collagenase‐dispersed pancreatic fragments. Thirty‐three normal animals acted as controls. Blood glucose, lactate, pyruvate, alanine, glycerol, 3‐hydroxybutyrate, cholesterol, free fatty acids, dinsulin, glucagon, and cortisol were determined in the fasting state and following an intravenous glucose load. At 1 month, glucose clearance (K) was reduced and there were elevated fasting levels of glycerol, cholesterol, free fatty acids, and 3‐hydroxybutyrate. At 3 months, significant improvements in K had occurred and intermediary metabolism returned to normal. There was a significant increase in glucosestimulated insulin output measured in peripheral blood between 1 and 3 months. Profiles for glucagon and cortisol were normal throughout the study.Unlike segmental pancreatic transplants, the pancreatic islet graft demonstrates functional improvement with time, a feature of considerable importance for future clinical studies.