Prevalence and molecular characterization of chloramphenicol resistance in Riemerella anatipestifer isolated from ducks and geese in Taiwan
Prevalence and molecular characterization of chloramphenicol resistance in Riemerella anatipestifer isolated from ducks and geese in Taiwan
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DOI：
10.1080/03079457.2010.507761
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发表时间：
2010-01-01
期刊：
影响因子：
2.8
通讯作者：
Tsai, Hsiang-Jung
中科院分区：
文献类型：
--
作者：
Chen, Yen-Ping;Tsao, Ming-Yang;Tsai, Hsiang-Jung
鸭疫里默氏杆菌是一种革兰氏阴性细菌，可在广泛的野生和家养鸟类，特别是水禽中引起疾病。在R.目前尚未发现鸭疫病毒，需要进行调查。在本研究中，40.5%的R.结果显示，2003年以来，45.9%的鸭瘟病菌对氯霉素有抗药性，而13.5%的鸭瘟病菌对氯霉素敏感，氯霉素是一种台湾自2003年起禁止在食用动物中使用的抗生素。通过文库测序，鉴定出该抗性基因为cat基因。台湾红毛鼠中cat基因的流行率。鸭疫分离株检出率为78.4%。然后确定cat基因在命名为pRA 0511的新质粒中的位置。对pRA 0511进行测序，显示其大小为11，435 bp，具有10个开放阅读框（ORF）。这10个开放阅读框编码的蛋白包括4个耐药相关蛋白。两个设计为氯霉素乙酰转移酶（CAT）的蛋白由两个不相邻的ORF编码，另外两个是TetX 2和多药物ABC转运蛋白通透酶/ATP酶。pRA 0511质粒是第一个在红曲霉中鉴定的耐药质粒，与已知的B型CAT有62.9 ~ 79.5%的同源性。anatipestifer，更具体地与氯霉素抗性相关。
Riemerella anatipestifer is a Gram-negative bacterium that can cause disease in a wide range of wild and domesticated birds, especially waterfowl. The presence of an antibiotic-resistance gene in R. anatipestifer has not yet been reported, indicating the need for investigation. In the present study, 40.5% of R. anatipestifer isolates were found to exhibit resistance to chloramphenicol, while 45.9% showed intermediate resistance and 13.5% were susceptible to chloramphenicol, an antibiotic that has been prohibited for use in food animals in Taiwan since 2003. The resistance gene was identified as the cat gene and cloned by library sequencing. The prevalence of the cat gene in Taiwanese R. anatipestifer isolates was 78.4%. The position of the cat gene was then determined within the novel plasmid, designated pRA0511. pRA0511 was sequenced and shown to be 11,435 bp in size with 10 open reading frames (ORFs). Proteins putatively encoded by these 10 ORFs included four drug-resistance-associated proteins. Two proteins designed as chloramphenicol acetyltransferases (CATs) were encoded by two non-adjacent ORFs, and the other two were TetX2 and a multi-drug ABC transporter permease/ATPase. The putative CAT protein had 62.9 to 79.5% homology to a known type B CAT. The pRA0511 plasmid is the first identified drug-resistance plasmid in R. anatipestifer, more specifically associated with chloramphenicol resistance.