OsMADS27 regulates the root development in a NO3--Dependent manner and modulates the salt tolerance in rice (Oryza sativa L.)
OsMADS27 regulates the root development in a NO3--Dependent manner and modulates the salt tolerance in rice (Oryza sativa L.)
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OsMADS27 以 NO3 依赖性方式调节根部发育并调节水稻 (Oryza sativa L.) 的耐盐性
DOI：
10.1016/j.plantsci.2018.09.004
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发表时间：
2018-12-01
期刊：
影响因子：
5.2
通讯作者：
Jin, Liang
中科院分区：
文献类型：
--
作者：
Chen, Hongli;Xu, Ning;Jin, Liang
OsMADS27是水稻中anr1样同源物之一，但其在植物生长发育及非生物胁迫应答中的功能尚不清楚。在这里，我们研究了OsMADS27在响应NO3-可用性的根发育中的作用。OsMADS27的组成性表达显著抑制了主根（PR）的伸长，但以依赖NO3的方式促进了侧根（LR）的形成。此外，OsMADS27的过表达促进了NO3-的积累以及NO3-转运基因的表达。ABA在介导NO3-对根发育的影响中发挥重要作用，因此推测OsMADS27可能通过ABA途径调控根的生长发育。OsMADS27过表达系的根生长发育对外源ABA的敏感性高于野生型。此外，在NO3-条件下，OsMADS27过表达植株的ABA积累水平较高。酵母双杂交和双分子荧光互补（BiFC）实验表明，OsMADS27通过DELLA蛋白OsSLR1与ABA-INSENSITIVE5 （OsABI5）发生物理相互作用。更重要的是，OsMADS27过表达可以增强耐盐性。综上所述，我们的研究结果表明OsMADS27是水稻根系发育和对渗透胁迫适应的重要调控因子。
OsMADS27 is one of the ANR1-like homologues in rice, whreas its functions in plant growth and development as well as the abiotic stress responses remain unclear. Here we investigated the roles of OsMADS27 in the root development in response to NO3- availability. Constitutive expression of OsMADS27 significantly inhibited the elongation of primary root (PR), but enhanced lateral root (LR) formation in a NO3- dependent manner. Furthermore, OsMADS27 overexpression promoted NO3- accumulation as well as the expression of NO3- transporter genes. ABA is reported to play an important role in mediating the effects of NO3- on the root development, thus it is supposed that OsMADS27 might regulate the root growth and development by ABA pathway. The root growth and development in OsMADS27 overexpression lines was shown to be more sensitive to exogenous ABA than wild type. Moreover, under NO3- conditions, higher levels of ABA accumulates in OsMADS27 overexpression plants. Yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays showed that OsMADS27 physically interacts with ABA-INSENSITIVE5 (OsABI5) via DELLA protein OsSLR1. More importantly, OsMADS27 overexpression could enhance the salt tolerance. Taken together, our findings suggested that OsMADS27 is an important regulator controlling the root system development and adaption to osmotic stress in rice.