Lack of relationship between 3′, 5′cyclic adenosine monophosphate desensitization and thyrotropin receptor down regulation in the rat thyroid cell line FRTL5
Lack of relationship between 3′, 5′cyclic adenosine monophosphate desensitization and thyrotropin receptor down regulation in the rat thyroid cell line FRTL5
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大鼠甲状腺细胞系 FRTL5 中 3, 5环单磷酸腺苷脱敏与促甲状腺素受体下调之间缺乏关系
DOI：
10.1007/bf03346791
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发表时间：
1991
影响因子：
5.4
通讯作者：
S. Filetti
中科院分区：
文献类型：
--
作者：
Daniela Foti;R. Catalfamo;D. Russo;G. Costante;S. Filetti
我们研究了慢性暴露于TSH后FRTL 5大鼠甲状腺细胞系的cAMP反应和放射性碘标记的TSH结合。在平行实验中，连续暴露于TSH诱导细胞cAMP反应性降低的第二次急性刺激促甲状腺激素（脱敏）和减少125 I-TSH细胞结合（下调）。这两个过程似乎不同的是：a）cAMP脱敏是最大的（与对照细胞相比，抑制率为45-55%）;相反，仅在24小时后检测到TSH与甲状腺细胞结合的损失，并且是最大的（比对照细胞低55-65%）; B）观察到cAMP脱敏和TSH下调对TSH剂量的不同依赖性：前者在TSH浓度为10 μU/ml时最大，后者仅在TSH浓度为10 mU/ml时最大; c）在TSH停药后3小时检测到cAMP脱敏的恢复，并在24小时完全达到，而从TSH受体下调的恢复在长达24小时内不明显，并且仅在72小时获得完全恢复; d）cAMP类似物的使用证明，与脱敏不同，TSH受体下调是cAMP依赖性过程。这些研究结果支持的概念，不同的模式响应长期刺激TSH，早期和cAMP的独立过程（脱敏）和后期cAMP依赖性机制（下调），发生在大鼠甲状腺细胞。
We studied the cAMP response and radioiodinated TSH binding in the FRTL5 rat thyroid cell line after chronic exposure to TSH. In parallel experiments, continuous exposure to TSH induced both a decrease in the cellular cAMP responsiveness to a second acute stimulation by thyrotropin (desensitization) and a reduction in 125I-TSH cellular binding (down-regulation). These two processes appeared to differ in that: a) cAMP desensitization was maximal (45–55% inhibition in comparison to control cells) after 24 h of preexposure to TSH; on the contrary, a loss of the TSH binding to thyroid cells was detected only after 24 h and was maximal (55–65% lower than control cells) after 48 h of preexposure to TSH; b) a different dependence on the TSH dose was observed for cAMP desensitization and TSH down-regulation: the former was maximal at 10 µU/ml of TSH, the latter only at 10 mU/ml of TSH; c) recovery from cAMP desensitization was detected 3 h after TSH withdrawal and was fully achieved at 24 h, whereas the recovery from the TSH receptor down-regulation was not evident for up to 24 h and a full recovery obtained only at 72 h; d) the use of cAMP analogues proved that, unlike desensitization, TSH receptor down-regulation is a cAMP dependent process. These findings support the concept that different patterns of response to prolonged stimulation with TSH, an earlier and cAMP-independent process (desensitization) and a later cAMP-dependent mechanism (down-regulation), occur in the rat thyroid cell.
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通过烟酰胺预防培养的人甲状腺细胞对促甲状腺素刺激的脱敏。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Filetti,S;Takai,NA;Rapoport,B
通讯作者：
Rapoport,B
环磷酸腺苷（而非黄体酮）在颗粒细胞黄体生成素受体下调中发挥核心作用。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Schwall,RH;Erickson,GF
通讯作者：
Erickson,GF
钙在诱导 TSH 对环 AMP 刺激不应性中的作用。
DOI：
10.1016/0026-0495(85)90158-1
发表时间：
1985
期刊：
Metabolism: clinical and experimental
影响因子：
--
作者：
Chayoth,R;Arem,R;Yoshimura,Y;Field,JB
通讯作者：
Field,JB
促甲状腺素诱导的牛甲状腺腺苷酸环化酶脱敏对核苷酸调节蛋白的影响。
影响因子：
4.8
作者：
Totsuka,Y;Nielsen,TB;Field,JB
通讯作者：
Field,JB
FRTL-5 大鼠甲状腺滤泡细胞系中胰岛素样生长因子-I 受体的表征和调节。
影响因子：
4.8
作者：
Tramontano,D;Moses,AC;Picone,R;Ingbar,SH
通讯作者：
Ingbar,SH