Specular microscopy ancillary study methods for donor endothelial cell density determination of cornea donor study images
Specular microscopy ancillary study methods for donor endothelial cell density determination of cornea donor study images
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DOI：
10.1080/02713680500536738
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发表时间：
2006-04-01
影响因子：
2
通讯作者：
Lass, JH
中科院分区：
文献类型：
--
作者：
Benetz, BA;Gal, RL;Lass, JH
目的：描述在多中心眼库研究中确定中央角膜内皮细胞密度(ECD)的可靠方法。方法：对于角膜供体研究中的镜检辅助研究中获得的供体内皮细胞图像的子集，镜面显微镜阅读中心采用双重分级程序和评判程序对图像质量进行分类和确定ECD。(1)两个认证阅读器将图像分类为可分析的(优秀、良好、一般)或不可分析的图像，并使用可变框架技术确定ECD。如果两个读取器的质量分类相差一个等级，如果任何一个读取器发现图像无法分析，和/或如果两个读取器之间的ECD确定为&>=5%，则裁判员也对图像进行评估。结果：两种阅读器对688张供体图像中的441张图像质量分类完全一致(%)。ECD不同之处在于
Purpose: To describe reliable methods for determining central corneal endothelial cell density (ECD) in a multicenter eye bank study. Methods: The Specular Microscopy Reading Center utilized a dual-grading procedure and adjudication process to classify image quality and determine ECD for a subset of donor endothelial images obtained in the Specular Microscopy Ancillary Study, which is part of the Cornea Donor Study.(1) Two certified readers classified images as analyzable (excellent, good, fair) or unanalyzable and determined the ECD using a variable frame technique. An adjudicator also evaluated the images if quality classifications by the two readers differed by one grade, if any reader found the image unanalyzable, and/or if the ECD determination between the two readers was >= 5%. Results: Image quality categorization by the two readers was identical for 441 (64%) of 688 donor images. The ECD differed by