CHANNEL OPEN TIME OF ACETYLCHOLINE-RECEPTORS ON XENOPUS MUSCLE-CELLS IN DISSOCIATED CELL-CULTURE
CHANNEL OPEN TIME OF ACETYLCHOLINE-RECEPTORS ON XENOPUS MUSCLE-CELLS IN DISSOCIATED CELL-CULTURE
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DOI：
10.1016/0012-1606(82)90012-4
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发表时间：
1982-01-01
影响因子：
2.7
通讯作者：
KIDOKORO, Y
中科院分区：
文献类型：
--
作者：
BREHM, P;STEINBACH, JH;KIDOKORO, Y
用聚焦记录的膜电流研究了非洲爪蟾胚胎肌节细胞乙酰胆碱（ACh）受体通道的动力学。在分离的细胞培养物中生长的胚胎肌细胞上的受体通道的平均开放时间显示出与在体内观察到的相似的时间依赖性降低。功率密度谱的变化表明，减少的结果从一类ACh受体的平均通道开放时间短（0.7 ms）的出现和减少的受体的比例与平均通道开放时间长（3 ms）。此外，解离的神经管细胞的肌肉细胞培养物中的ACh受体在突触和nonsynaptic地区的平均通道开放时间意外增加。乙酰胆碱受体功能可能会改变培养的肌肉细胞。
The kinetics of acetylcholine (ACh) receptor channels on cultured myotomal muscle cells from Xenopus embryos were studied by analyzing focally recorded membrane currents. The mean open time for receptor channels on embryonic muscle cells grown in dissociated cell cultures showed a time-dependent decrease similar to that seen in vivo. The changes in power density spectra indicate that the decrease results from the appearance of a class of ACh receptor with a short mean channel open time (0.7 ms) and a decrease in the proportion of receptors with a long mean channel open time (3 ms). Addition of dissociated neural tube cells to muscle cell cultures resulted in an unexpected increase in mean channel open time for ACh receptors in synaptic and nonsynaptic regions. ACh receptor function may be altered in cultured muscle cells.