Effects of changes in ionic environment on the action of acetylcholine and adrenaline on the smooth muscle cells of guinea‐pig taenia coli
Effects of changes in ionic environment on the action of acetylcholine and adrenaline on the smooth muscle cells of guinea‐pig taenia coli
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离子环境变化对乙酰胆碱和肾上腺素对豚鼠大肠杆菌平滑肌细胞作用的影响
DOI：
10.1113/jphysiol.1963.sp007090
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发表时间：
1963
期刊：
影响因子：
--
通讯作者：
H. Kuriyama
中科院分区：
文献类型：
--
作者：
E. Bülbring;H. Kuriyama
Builbring（1955,1957）描述了乙酰胆碱和肾上腺素对带绦虫线圈单细胞活性的作用：乙酰胆碱使膜去极化，增加尖峰频率，延长尖峰持续时间。肾上腺素减缓或消除尖峰放电，缩短动作电位持续时间，引起膜电位升高。Burnstock （1958a, b）用蔗糖间隙法证实并扩展了这些观察结果。他讨论了乙酰胆碱在Na+和K+离子跨膜运动中的作用，并将其与在骨骼肌终板区域的作用进行了比较。他还推测肾上腺素产生的膜电位的增加可能是由于电致钠泵的刺激，以及抑制尖峰活性对钠或其他离子载体机制的抑制。Bulbring & Burnstock（1960）利用蔗糖间隙法研究了乙酰胆碱、组胺和5-羟色胺对大肠带绦虫膜活性的加速反应和增强作用的条件。他们将这两种现象解释为与活性离子转运率和代谢能量利用率有关。Axelsson, buding & Bulbring（1961）使用生化和电生理方法观察了肾上腺素的作用。他们得出结论，肾上腺素对细胞膜的超极化和稳定化与磷酸化酶活性的增加有因果关系（参见Bulbring， 1960）。*乙酰胆碱和肾上腺素对大肠带菌单细胞活性的影响类似于对子宫肌的影响（Marshall, 1959; Kuriyama, 1961），类似于对兔结肠的副交感神经和交感神经的刺激（Gillespie, 1961 a, b）。这里所描述的工作是为了查明是否
The action of acetylcholine and adrenaline on single cell activity of the taenia coil has been described by Builbring (1955, 1957): Acetylcholine depolarizes the membrane, increases the spike frequency and prolongs the spike duration. Adrenaline slows or abolishes the spike discharge, reduces the duration of the action potential and causes a rise of membrane potential. These observations were confirmed and expanded by Burnstock (1958a, b) using the sucrose-gap method. He discussed the action of acetylcholine in relation to the movement of Na+ and K+ ions across the membrane and compared it with its action on the end-plate region of skeletal muscle. He also speculated that the increase of membrane potential produced by adrenaline might be due to the stimulation of an electrogenic sodium pump, and the inhibition of spike activity to an inhibition of the carrier mechanism for sodium or some other ion. Bulbring & Burnstock (1960), using the sucrose-gap method, investigated the conditions causing tachyphylaxis and potentiation of the effects of acetylcholine, histamine and 5-hydroxytryptamine on the membrane activity of taenia coli. They interpreted these two phenomena in relation to the rates of active ion transport and utilization of metabolic energy. Axelsson, Bueding & Bulbring (1961) observed the effect of adrenaline using both biochemical and electrophysiological methods. They came to the conclusion that the hyperpolarization and stabilization of the membrane by adrenaline had a causal relationship to the increase of phosphorylase activity (see also Bulbring, 1960).* The effects of acetylcholine and adrenaline on single cell activity in taenia coli are similar to those described in uterine muscle (Marshall, 1959; Kuriyama, 1961) and to those of parasympathetic and sympathetic nerve stimulation in rabbit colon (Gillespie, 1961 a, b). The work to be described here was undertaken to find out whether the