Structure and localization of cyclosporin synthetase, the key enzyme of cyclosporin biosynthesis in Tolypocladium inflatum
Structure and localization of cyclosporin synthetase, the key enzyme of cyclosporin biosynthesis in Tolypocladium inflatum
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DOI：
10.1007/s002030100324
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发表时间：
2001-10-01
影响因子：
2.8
通讯作者：
Schörgendorfer, K
中科院分区：
文献类型：
--
作者：
Hoppert, M;Gentzsch, C;Schörgendorfer, K
天然环孢菌素合成酶分子的电子显微镜图像显示直径为 25 nm 的大型球状复合物，由较小的互连单元组成。对亚细胞级分进行蔗糖密度梯度离心和免疫电子显微镜检查后，揭示了环孢菌素合成酶和功能上相互连接的 D-丙氨酸消旋酶的区室。在液泡膜上检测到相当比例的环孢菌素合成酶和D-丙氨酸消旋酶。产物环孢菌素定位于真菌液泡中。
The electron microscopic image of native cyclosporin synthetase molecules showed large globular complexes of 25 nm in diameter, built up by smaller interconnected units. Compartmentation of cyclosporin synthetase and the functionally interconnected D-alanine racemase was revealed after sucrose density gradient centrifugation of subcellular fractions and immunoelectron microscopy. A considerable proportion of cyclosporin synthetase and D-alanine racemase was detected at the vacuolar membrane. The product cyclosporin was localized in the fungal vacuole.