Graphene@AuNPs modified molecularly imprinted electrochemical sensor for the determination of colchicine in pharmaceuticals and serum
Graphene@AuNPs modified molecularly imprinted electrochemical sensor for the determination of colchicine in pharmaceuticals and serum
复制标题
石墨烯@AuNPs修饰的分子印迹电化学传感器用于测定药物和血清中的秋水仙碱
DOI：
10.1016/j.jelechem.2018.02.061
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发表时间：
2018-05-01
影响因子：
4.5
通讯作者：
Cao, Qiue
中科院分区：
文献类型：
--
作者：
Bai, Huiping;Wang, Chunqiong;Cao, Qiue
在本研究中，基于石墨烯/金纳米颗粒（G@AuNPs）复合材料和分子印迹膜（MIM）修饰的玻璃碳电极（GCEs）开发了一种分子印迹电化学传感器，用于药物和血清中秋水仙碱的灵敏检测（MIM/G@AuNPs/GCE）。以甲基丙烯酸为功能单体，在G@AuNPs-modified GCE上原位聚合秋水仙碱印迹膜。由于G@AuNPs复合材料的形成，电极的比表面积、催化性能和电导率都有所提高，从而提高了传感器的灵敏度。作为预富集剂的MIM表现出化学选择性。在最优条件下，MIM/G@AuNPs/GCE传感器具有较高的选择性和抗干扰能力。秋水仙碱在1.2 × 10(-8) ~ 1.2 × 10(-6) mol L-1和1.2 × 10(-6) ~ 1.0 × 10(-4) mol L-1范围内呈良好的线性关系，检出限为4.8 × 10(-9) mol L-1。该传感器可用于直接测定秋水仙碱片和人血清样品中的秋水仙碱，相对标准偏差小于3.1% (n = 5)，加样回收率为97.5% ~ 110.0%。
In this study, a molecularly imprinted electrochemical sensor based on graphene/Au nanoparticles (G@AuNPs) compound materials and molecularly imprinted membrane (MIM) modified glassy carbon electrodes (GCEs) was developed for the sensitive detection of colchicine in pharmaceuticals and serum (MIM/G@AuNPs/GCE). The film was formed by the in situ polymerization of colchicine-imprinted membranes on the G@AuNPs-modified GCE using methacrylic acid as the functional monomer. The specific surface area, catalytic performance, and electrical conductivity of the electrode increased on account of the formation of G@AuNPs compound materials, thereby improving the sensitivity of the sensor. The MIM as a preconcentrator exhibited chemical selectivity. Under optimal conditions, the MIM/G@AuNPs/GCE sensor exhibited high selectivity and high anti-interference ability. Moreover, good linearity was obtained in the range of 1.2 x 10(-8) to 1.2 x 10(-6) mol L-1 and 1.2 x 10(-6) to 1.0 x 10(-4) mol L-1 of colchicine with a detection limit of 4.8 x 10(-9) mol L-1. The sensor was successfully applied for the direct determination of colchicine in colchicine tablets and human serum samples, with a relative standard deviation of less than 3.1% (n = 5) and recoveries ranging from 97.5% to 110.0%.