Enhanced osteoinduction by controlled release of bone morphogenetic protein-2 from biodegradable sponge composed of gelatin and β-tricalcium phosphate
Enhanced osteoinduction by controlled release of bone morphogenetic protein-2 from biodegradable sponge composed of gelatin and β-tricalcium phosphate
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DOI：
10.1016/j.biomaterials.2005.01.012
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发表时间：
2005-08-01
期刊：
影响因子：
14
通讯作者：
Tabata, Y
中科院分区：
文献类型：
--
作者：
Takahashi, Y;Yamamoto, M;Tabata, Y
制备了不同P-磷酸三钙（β-TCP）含量的生物可降解明胶海绵，以允许骨形态发生蛋白（BMP）-2掺入其中。研究了掺入BMP-2的海绵的体内骨诱导活性，同时评价了它们的体内BMP-2释放概况。所制备的海绵具有平均孔径为200 μ m的互连孔结构，与β-TCP含量无关。体内释放试验表明，BMP-2在体内以相似的时间曲线释放，而与β-TCP含量无关。BMP-2在体内的保留时间超过28天。当明胶或明胶-β-TCP海绵纳入BMP-2的骨诱导活性进行了研究植入到大鼠背部皮下组织的组织学和生物化学检查，均匀的骨形成组织学观察整个海绵，虽然骨形成的程度较高的海绵与较低的β-TCP的内容。另一方面，碱性磷酸酶活性和骨钙素含量的水平在植入部位的海绵下降的β-TCP含量的增加。明胶海绵表现出显着更高的骨诱导活性比任何明胶-β-TCP海绵，虽然每一个海绵有或没有β-TCP显示出类似的体内BMP-2释放的配置文件。此外，体外胶原酶消化实验表明，明胶-β-TCP海绵比没有β-TCP掺入的明胶海绵更容易塌陷。这些结果表明，维持骨诱导所必需的海绵内空间是促进BMP-2掺入海绵的骨诱导程度的一个因素。(C)2005爱思唯尔有限公司保留所有权利。
Biodegradable gelatin sponges at different contents of P-tricalcium phosphate (beta-TCP) were fabricated to allow bone morphogenetic protein (BMP)-2 to incorporate into them. The in vivo osteoinduction activity of the sponges incorporating BMP-2 was investigated, while their in vivo profile of BMP-2 release was evaluated. The sponges prepared had an interconnected pore structure with an average pore size of 200 mu m, irrespective of the beta-TCP content. The in vivo release test revealed that BMP-2 was released in vivo at a similar time profile, irrespective of the beta-TCP content. The in vivo time period of BMP-2 retention was longer than 28 days. When the osteoinduction activity of gelatin or gelatin-beta-TCP sponges incorporating BMP-2 was studied following the implantation into the back subcutis of rats in terms of histological and biochemical examinations, homogeneous bone formation was histologically observed throughout the sponges, although the extent of bone formation was higher in the sponges with the lower contents of beta-TCP. On the other hand, the level of alkaline phosphatase activity and osteocalcin content at the implanted sites of sponges decreased with an increase in the content of beta-TCP. The gelatin sponge exhibited significantly higher osteoinduction activity than that of any gelatin-beta-TCP sponge, although every sponge with or without beta-TCP showed a similar in vivo profile of BMP-2 release. In addition, the in vitro collagenase digestion experiments revealed that the gelatin-beta-TCP sponge collapsed easier than the gelatin sponge without beta-TCP incorporation. These results suggest that the maintenance of the intrasponge space necessary for the osteoinduction is one factor contributing to the osteoinduction extent of BMP-2-incorporating sponges. (C) 2005 Elsevier Ltd. All rights reserved.