Positive correlation of skeletal muscle UCP3 mRNA levels with overweight in male, but not in female, rats.
Positive correlation of skeletal muscle UCP3 mRNA levels with overweight in male, but not in female, rats.
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DOI：
10.1152/ajpregu.00698.2002
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发表时间：
2003-10
期刊：
影响因子：
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通讯作者：
Ana M Rodríguez;P. Roca;M. Bonet;C. Picó;P. Oliver;A. Palou
中科院分区：
文献类型：
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作者：
Ana M Rodríguez;P. Roca;M. Bonet;C. Picó;P. Oliver;A. Palou
本研究的目的是探讨骨骼肌解偶联蛋白（UCP）3 mRNA表达的性别依赖性调节超重及其与血清游离脂肪酸，瘦素和胰岛素水平的关系。使用了两种肥胖模型：用自助餐喂养大鼠14周使其肥胖，自助餐后超重大鼠在食用自助餐14周后再喂普通饲料10周。在postcafetan大鼠及其年龄匹配的对照组中研究了24小时禁食的影响。自助餐厅大鼠食用高脂肪饮食，体重超标，雌性（+59%）高于雄性（+39%）。自助餐饮食后，雄性大鼠的腹肌UCP 3 mRNA诱导水平明显高于雌性大鼠（增加+116% vs增加+26%）。Postcafetan雄性大鼠（而非雌性大鼠）仍显示出相对于其年龄匹配的对照组UCP 3 mRNA水平增加的趋势。线性回归分析显示UCP 3 mRNA水平与超重、血清瘦素和胰岛素水平呈显著正相关，而与血清游离脂肪酸水平无相关性。随后的相关性分析和多元线性回归分析表明，超重是唯一的参数实际上与UCP 3 mRNA水平的男性。禁食诱导的肌肉UCP 3 mRNA水平上调在雄性（5- 7倍）高于雌性（3- 4倍）。我们的研究结果表明，在控制肌肉UCP 3表达方面存在性别相关差异，以应对超重和禁食，在这两种情况下，雌性大鼠的诱导受损。腹部肌肉UCP 3 mRNA的表达与超重的男性可能与他们的相对阻力增加体重后，慢性过度饮食的自助餐饮食，UCP 3在调节脂质利用的作用。
The objective of this study was to investigate the sex-dependent regulation of skeletal muscle uncoupling protein (UCP)3 mRNA expression in response to overweight and its relationship with serum levels of free fatty acids, leptin, and insulin. Two obesity models were used: rats made obese by feeding them with a cafeteria diet for 14 wk, and postcafeteria overweight rats fed a chow diet for 10 wk after consuming the cafeteria diet for 14 wk. The effects of 24-h fasting were studied in postcafeteria rats and their age-matched controls. The cafeteria rats ate a high-fat diet and attained an excess body weight that was higher in females (+59%) than in males (+39%). A trend to higher induction of abdominal muscle UCP3 mRNA in male rats than in females after cafeteria diet was apparent (+116% increase vs. +26% increase). Postcafeteria male but not female rats still showed the tendency to have increased UCP3 mRNA levels relative to their age-matched controls. A linear regression analysis showed a significant positive correlation of the UCP3 mRNA levels with overweight and with serum levels of leptin and insulin in males, but not in females, and no correlation with serum free fatty acid levels. A subsequent correlation analysis and a multiple linear regression analysis showed that overweight was the only parameter actually related to UCP3 mRNA levels in males. Fasting-induced upregulation of muscle UCP3 mRNA levels was higher in males (5- to 7-fold) than in females (3- to 4-fold). Our results point to the existence of sex-associated differences in the control of muscle UCP3 expression in response to overweight and fasting, with an impaired induction in female rats under both conditions. The correlation of abdominal muscle UCP3 mRNA expression with overweight in males could be related to their relative resistance to gain weight after chronic overeating of a cafeteria diet, by the purported role of UCP3 in the regulation of lipid utilization.